蒙古冰草(Agropyron mongolicum)落粒相关基因Amsh1克隆及进化分析  

Cloning and Evolutionary Analysis of Shattering Related Gene Amsh1 in Agropyron mongolicum

作　　者：田青松[1] 高金玉[2] 融晓萍 张瑞霞 韩冰[1,2] 索培芬[2] 李晓全[2] Tian Qingsong;Gao Jinyu;Rong Xiaoping;Zhang Ruixia;Han Bing;Suo Peifen;Li Xiaoquan(Institute of Grassland Research of CAAS,Hohhot,010010;College of Life Sciences,Inner Mongolia Agricultural University,Hohhot,010018;Inner Mongolia Agricultural Technology Extension Station,Hohhot,010011;Hohhot Seed Management Station,Hohhot,010011)

机构地区：[1]中国农业科学院草原研究所,呼和浩特010010 [2]内蒙古农业大学生命科学学院,呼和浩特010018 [3]内蒙古自治区农业技术推广站,呼和浩特010011 [4]呼和浩特市种子管理站,呼和浩特010020

出　　处：《分子植物育种》2018年第22期7289-7297,共9页Molecular Plant Breeding

基　　金：西部之光人才培养计划项目;内蒙古自然科学基金项目(2015MS0305)共同资助

摘　　要：蒙古冰草因籽粒脱落导致的种子减产现象非常严重。为了揭示蒙古冰草的落粒机制,本研究主要通过同源克隆的方法,扩增得到蒙古冰草落粒相关基因的cDNA全长序列,分别命名为Amsh1-1和Amsh1-2。对克隆到的基因进行生物信息学分析。结果表明,Amsh1-1基因cDNA全长935 bp,开放阅读框561 bp,共编码氨基酸186个,蛋白质相关分子量为20 990.03,具有典型的YABBY结构域,是一个不稳定蛋白。Amsh1-2基因cDNA全长1 118 bp,开放阅读框624 bp,共编码氨基酸207个,蛋白质相关分子量为22 759.70,具有典型的YABBY结构域,是一个不稳定蛋白。系统进化树分析显示,Amsh1-1与大麦的Hvsh1同源性最高,Amsh1-2与毛竹的Pesh1同源性最高。本研究为进一步的探究落粒基因的作用机制提供理论依据。Agropyron mongolicum seed production reduction caused by the phenomenon of shattering is very serious. In order to reveal the mechanism of shattering in Agropyron mongolicum, we cloned the genes controlling the rate of shattering by homologous cloning in this study. Two full-length cDNAs of shattering genes were obtained, which were named as A mshl-1 and Am shl-2, respectively. Bioinformatics analysis of the obtained genes were carried out. The results showed that the full-length cDNA of Amshl-1 was 935 bp, with an open reading frame of 561 bp. It encoded a protein composed of 186 amino acids with a calculated molecular mass of 20 990.03. It was an unstable protein with a typical YABBY domain. While the full-length cDNA of A rash1-2 was 1 118 bp, with an open reading frame of 624 bp. It encoded a protein composed of 207 amino acids with a calculated molecular mass of 22 759.70. It was an unstable protein with a typical YABBY domain. Phylogenetic tree analysis indicated that A mshl-1 had the highest homology with Hvshl gene in Hordeum vulgare and A rash1-2 had the highest homology with Peshl gene in Phyllostachys edulis. This study would provide a theoretical basis for further exploring the mechanism of shattering genes.

关 键 词：落粒相关基因 Amsh1-1 Amsh1-2 蒙古冰草 同源克隆 

分 类 号：S543.9[农业科学—作物学]