正交法制备羧甲基壳聚糖-纳米银纳米微球  

Preparation of carboxymethyl chitosan-nano sliver nanoparticles by orthogonal design

作　　者：史梦柔 娄建石[1] 李光远[1,2] 郑夺[2] 陈斌[2] 高萍[2] 李毅敏[2] SHI Meng-rou;LOU Jian-shi;LI Guang-yuan;ZHENG Duo;CHEN Bin;GAO Ping;LI Yi-min(Department of Pharmacology,Tianjin Medical University,Tianjin 300070,China;Medical biological material supervision and research center,Tianjin Institute of Medical and Pharmaceutical Sciences,Tianjin 300020,China)

机构地区：[1]天津医科大学药理学教研室,天津300070 [2]天津市医药科学研究所医用生物材料监测研究中心,天津300020

出　　处：《天津医科大学学报》2018年第6期496-500,共5页Journal of Tianjin Medical University

基　　金：天津市卫生和计划生育委员会科技基金(2013KY38)

摘　　要：目的:制备以纳米银为模型药物的羧甲基壳聚糖载药纳米微球,探索载药纳米微球的制备条件对粒径、包封率的影响,确定最佳制备工艺。方法:三聚磷酸钠为交联剂,采用离子交联法制备载药纳米微球,测量药物的包封率及粒径。紫外分光光度法测定该制剂中纳米银释放情况。结果:通过正交实验设计,优化了制备工艺条件,其最佳条件是羧甲基壳聚糖浓度4.2 mg/mL,滴速为5 s/滴,纳米银投药量为20 mg(500μL)。在此条件下进行实验,制备出的载药纳米微球的平均粒径为514.1 nm,多分散系数0.204,包封率61.67%。8 h内累计释放量达80%以上,方程拟合以一级动力学方程拟合效果最好。结论:该制备工艺简单、稳定,可制备出包封率高、粒径适宜的羧甲基壳聚糖-纳米银纳米微球。Objective： To prepare carboxymethyl chitosan drug-loaded nanoparticles with nano silver as model drug the influence of the drug-loaded nanoparticles preparing conditions on the particle size and the encapsulation efficiency to select the optimum conditions for preparation. Methods：With sodium tripolyphosphate as crosslinking agent, Drug-loaded nanoparticles were prepared by ionic cross- Linking method. The drug encapsulation efficiency and particle size were measured. The release of nano-sliver from preparations byultraviolet spectrophotometry was observed. Results：Through the orthogonal design,preparation method was optimized. The optimum conditions included the carboxymethyl chitosan concentration of 4.2 mg/mL,speed of 5 s/drops, and the best dosage of nano sliver for 20 mg （500 FL）. In this experiment,the mean size of the preparation drug-loaded nanoparticles was 514.1 nm with the polydispersity index of 0.204,and the encapsulation efficiency was 61.67%.The accumulated amount of release in the preparations is more than 80% at 8 h. Equation of in vitro release model fitted best to Kinetic equation. Conclusion：This preparation method is simple and stable. The carboxymethyl chitosan-nano sliver nanoparticles can be prepared with a high encapsulation efficiency and suitable particle size.

关 键 词：羧甲基壳聚糖 纳米银 纳米微球 离子交联法 

分 类 号：R944.9[医药卫生—药剂学]