番鸭呼肠孤病毒感染对MPV-GPV弱毒疫苗抗体应答的影响  

Effect of muscovy duck reovirus infection on antibody response to MPV-GPV attenuated vaccine

作　　者：林锋强[1] 朱小丽[1] 陈仕龙[1] 肖世峰 程晓霞[1] 王劭[1] 陈少莺[1] Lin Fengqiang;Zhu Xiaoli;Chen Shilong;Xiao Shifeng;Cheng Xiaoxia;Wang Shao;Chen Shaoying(Institute of Animal Husbandry and Veterinary Medicine,Fujian Academy of Agriculture Sciences,Fujian Animal Diseases Control Technology Development Center,Fuzhou 350013)

机构地区：[1]福建省农业科学院畜牧兽医研究所福建省畜禽疫病防治工程技术研究中心,福州350013

出　　处：《福建畜牧兽医》2018年第6期7-10,共4页Fujian Journal of Animal Husbandry and Veterinary medicine

基　　金：福建省自然科学基金项目(2016J01137);福建省公益类科研院所专项(2016R1022-14)资助

摘　　要：1日龄番鸭感染番鸭呼肠孤病毒(MDRV)后,应用LPAI方法检测MPV-GPV二联弱毒疫苗免疫后抗体变化规律,应用MTT法测定番鸭血液、脾脏和胸腺的B淋巴细胞增殖功能,探讨MDRV对B淋巴细胞功能的影响。结果显示番鸭感染MDRV后免疫MPV-GPV二联疫苗,IM 7 d时番鸭GPV-LPAI效价平均值1.5log2;随后缓慢上升,到IM 21-28 d时GPVLPAI效价平均值维持在约3log2。与单独免疫组相比,GPV-LPAI效价大大降低,不能提供有效保护。1日龄番鸭人工感染MDRV后,PI 7 d外周血T淋巴细胞增殖指数与对照组相比明显下降(P<0.05)。PI 14-28 d与对照组相比下降,差异极显著(P<0.01),表明MDRV感染抑制了B淋巴细胞增殖功能。结果表明,MDRV感染能抑制抗体应答和番鸭外周血和免疫器官中B细胞的增殖功能作用,造成B细胞功能抑制。After 1-day-old muscovy ducks were infected with muscovy duck reovirus （MDRV）, the change of antibody after immunization with MPV-GPV bivalent attenuated vaccine was detected by LPAI method. The results showed that muscovy ducks were immunized with MPV-GPV vaccine after MDRV infection.The proliferation of B lymphocytes in blood, spleen and thymus of muscovy ducks was determined by MTT method. the average GPV-LPAI antiboy titer of muscovy ducks was 1.5 log2 at IM 7 d. Then slowly rise, and that average titer was maintain at about 3 log2 at IM 21-28 d. GPV-LPAI antiboy titer was significantly lower than that of the immunized group alone, which did not provide effective protection. After 1-day-old muscovy ducks were artificially infected with MDRV, the proliferation index of peripheral blood B lymphocyteswas significantly lower than that in control group P〈 0.05 in PI 7 d and P〈 0.01 in PI 14-28 days, showing that MDRV infection inhibited the proliferation of B lymphocytes. These results suggest that MDRV infection can inhibit antibody response and B cell proliferation, resulting in B cell function inhibition.

关 键 词：番鸭呼肠孤病毒 MPV-GPV疫苗 B细胞 抗体 增殖 

分 类 号：S858.32[农业科学—临床兽医学]