上皮间质转化相关蛋白在胆道闭锁肝纤维化中的表达研究  

Correlation between the expressions of epithelial-to-mesenchymal transition markers in children with biliary atresia

作　　者：吴艳[1] 杨文萍[1] 张守华[2] 徐红艳[1] 曾松涛[1] 黄慧[1] 熊枫[1] 曾华[1] Wu Yan;Yang Wenping;Zhang Shouhua;Xu Hongyan;Zeng Songtao;Huang Hui;Xiong Feng;Zeng Hua(Department of Pathology,Jiangxi Children＇s Hospital,Nanchang 330006,China;Department ofGeneral Surgery,Jiangxi Children＇s Hospital,Nanchang 330006,China)

机构地区：[1]江西省儿童医院病理科 [2]江西省儿童医院普外科,江西省南昌市330006

出　　处：《临床小儿外科杂志》2018年第11期826-829,共4页Journal of Clinical Pediatric Surgery

基　　金：江西省卫生计划与生育委员会科技计划(编号:20155586);国家自然科学基金项目(编号:81460118)

摘　　要：目的检测上皮间质转化相关蛋白(N-cadherin,E-cadherin,vimentin,SMA,CK19)在胆道闭锁(biliary atresia,BA)患儿肝组织中的表达情况,初步探讨其在BA患儿肝脏上皮间质转化中的意义。方法采用免疫组化法对34例BA患儿(实验组)及13例胆总管囊肿患儿(对照组)的肝组织标本进行上皮间质转化相关蛋白检测,分析其表达差异及其与肝纤维化严重度的相关性。结果实验组与对照组相比,N-cadherin、E-cadherin、vimentin、SMA、CK19抗体不同表达等级的构成比存在统计学差异(P <0. 05)。结论上皮间质转化相关蛋白在BA患儿肝组织中的表达水平总体高于胆总管囊肿患儿,提示上皮间质转化相关蛋白可能参与BA患儿肝纤维化的发生与发展。Objective To detect the expressions of N-cadherin E-cadherin vimentin SMA and CK19 inbiliary aressia and congenital cyst of common bile duct and discuss the relationship with in epithetialtomesenchymal transition. Methods The expression of N-cadherin,E-cadherin,vimentin,SMA and CK19 in biliaryatresia （n = 34） and common bile duct cyst （n = 13） were detected by immunohistochemistry SP method. Results The expression ratios of N-cadherin. E-cadherin,vimentin,SMA and CK19 were higher in liver of bileduct than those in common bile duct （P 〈 0. 05）. Conclusion The expressions of N-cadherin,vimentin,SMAand CK19 are closely correlated with epithelialtomesenchymal transition. And EMT may be one of importantcauses of hepatic cirrhosis.

关 键 词：胆道闭锁 肝硬化 免疫组织化学 研究 

分 类 号：R726.5[医药卫生—儿科]