28种AM真菌对滇重楼中薯蓣皂苷元含量的影响  

Effects of 28 Species of AM Fungi on Diosgenin Contents in Paris polyphylla var. yunnanensis

作　　者：王骞 张辉菊 杨敏 张华 周浓 李杨 Wang Qian;Zhang Huiju;Yang Min;Zhang Hua;Zhou Nong;Li Yang(College of Pharmacy and Chemistry,Dali University,Dali,Yunnan 671000,China;College of Food and Biological Engineering,Chongqing Three Gorges University,Chongqing 404120,China;The Chongqing Engineering Laboratory for Green Cultivation and Deep Processing of the Three Gorges Reservoir Area＇s Medicinal Herbs,Chongqing 404120,China;Editorial Department of Journal,Dali University,Dali,Yunnan 671003,China)

机构地区：[1]大理大学药学与化学学院,云南大理671000 [2]重庆三峡学院生物与食品工程学院,重庆404120 [3]三峡库区道地药材绿色种植与深加工重庆市工程实验室,重庆404120 [4]大理大学学报编辑部,云南大理671003

出　　处：《大理大学学报》2018年第10期22-25,共4页Journal of Dali University

基　　金：国家自然科学基金资助项目(81260622);云南省教育厅科学研究基金资助项目(2018JS413)

摘　　要：目的:通过室温盆栽接种试验,观察灭菌土壤条件下接种28种丛枝菌根(AM)真菌对滇重楼侵染率和薯蓣皂苷元含量的影响。方法:采用曲利本蓝染色法进行AM真菌的侵染率测定;采用HPLC方法测定滇重楼中薯蓣皂苷元含量。结果:滇重楼与AM真菌形成了良好的共生关系,并且侵染率显著提高;大部分AM真菌施用后滇重楼薯蓣皂苷元含量与对照组(CK组)比较差异无统计学意义(0.978 2～10.745 3 mg/g),但是Svi真菌处理后滇重楼薯蓣皂苷元含量显著提高(41.404 5 mg/g)。结论:AM真菌可与滇重楼形成良好的共生关系,并且侵染率显著提高;Svi真菌提高滇重楼薯蓣皂苷元含量效果显著。Objective： After 28 foreign species of AM fungi were inoculated in sterilized soil, the effects of the AM on mycorrhizal colonization and the diosgenin content of Paris polyphylla var. yunnanensis were observed by inoculation test in pot at room temperature. Methods： Trypan blue staining was used to test AM colonization, and HPLC was applied for analyzing diosgenin content. Results： The good symbiotic relationship between Paris polyphylla var. yunnanensis and AM fungi species was observed, and AM fungi species enhanced the colonization. Most AM fungi species did not ilffluence the diosgenin content （0.978 2-10.745 3mg/g） compared with the control group （CK）. However, Svi fungi significantly increased the diosgenin content （41.404 5 mg/g）. Conclusion： There are symbiotic relationships between arbuscular mycorrhizal （AM） fungi and Paris polyphylla var. yunnanensis. AM fungi species can enhance the colonization, and Svi fungi species can greatly increase the diosgenin content.

关 键 词：滇重楼 AM真菌 菌根侵染率 薯蓣皂苷元 

分 类 号：R284[医药卫生—中药学]