甘蓝型油菜显性核不育系的分子标记辅助选择  

Marker Assisted Selection of Dominant Genic Male Sterility Lines in Brassica napus L.

作　　者：张俊英[1] 蒋美艳[1] 江建霞 李延莉[1] 王伟荣[1] 孙超才[1] 杨立勇[1] 周熙荣[1] ZHANG Junying;JIANG Meiyan;JIANG Jianxia;LI Yanli;WANG Weirong;SUN Chaocai;YANG Liyong;ZHOU Xirong(Crop Breeding and Cultivation Research Institute,Shanghai Academy of Agricultural Sciences,Shanghai 201403)

机构地区：[1]上海市农业科学院作物育种栽培研究所,上海201403

出　　处：《核农学报》2018年第11期2119-2125,共7页Journal of Nuclear Agricultural Sciences

基　　金：上海市农委种业发展项目[沪农科种字(2015)第1号];上海市农委基础研究领域项目[沪农科攻字(2014)第7-1-1号];上海市农委青年人才成长计划[沪农青字(2018)第1-3号];上海市农业科学院2018年青年科技人员"助跑"计划[沪农科(2017)20号]

摘　　要：为探究分子标记BE10对甘蓝型油菜显性核不育系选育中的作用,本研究以甘蓝型油菜核不育系为材料,利用分子标记BE10,检测甘蓝型油菜显性核不育系的自交分离群体和纯合型不育株,并通过田间育性观察,验证分子标记BE10辅助选择的可靠性。结果表明,基因型为MS5~aMS5~b的可育株自交,其后代的育性表现为3∶1(可育∶不育),基因型分离结果为1MS5~aMS5~a:2MS5~aMS5~b:1MS5~bMS5~b。选取可育株自交,三分之二的单株后代分离比例与上一世代相同,三分之一的单株后代群体因携带纯合型的MS5~a而表现为100%可育。利用分子标记BE10的辅助选择,不仅能鉴定出后代群体是否有MS5~b不育基因,提高不育系的选育效率,还能从育性表现为3∶1(可育∶不育)的自交系中筛选出纯合型不育株;结合无性繁殖技术,用纯合型不育株的无性系与临保系生产全不育系,可有效提高全不育系的纯度,为杂交种选育和杂交种子生产提供了新思路和新途径。To investigated the function of molecular marker BE10 in breeding Brassica napus Dominant Genic Male Sterility（ DGMS） lines,using BE10 to detect the selfing segregation populations and the homozygous sterile lines of the of B. napus DGMS line and verity the reliability of BE10 was verified through field fertility observation. Selfing population of the fertile lines with the genotype MS5^aMS5^bgenerated a fertile/sterile segregation ratio of 3： 1,with genotypes MS5^aMS5^a（ 1） ： MS5^aMS5^b（ 2） ： MS5^bMS5^b（ 1）. From this population,the fertile lines were selected for self-pollination. Two-thirds of the resulting populations showed the same segregation ratio as the parental generation. The remaining populations, carrying the homozygous MS5^a,were completely fertile. The sterile gene MS5^band the homozygous sterile lines can be identified using the marker BE10 from the progeny population. This method could effectively improve the breeding efficiency of the sterile line. The selected homozygous sterile plants can be propagated with the aid of clonal reproduction. The resulting plants can be used to produce full sterile lines by crossing them with the temporary maintainer lines. This integrated approach can effectively improve the purity of full sterile lines and thus provide an efficient and reliable way for breeding and commercially producing male sterile hybrids.

关 键 词：甘蓝型油菜 显性核不育 分子标记辅助选择 

分 类 号：S565.4[农业科学—作物学]