不同饲料配方复制混合型高脂血症大鼠模型的实验研究  

Experimental Study of Combined Hyperlipidemia Rat Model Induced by Different Kinds of Diets

作　　者：严家荣[1] 吴贤生[1] 黎雄才[1] 刘盛来[1] 刘月姝[1] 邝少松[1] YAN Jiarong;WU Xiansheng;LI Xiongcai;LIU Shenglai;LIU Yueshu;KUANG Shaosong(Comparative Medical Laboratory,Guangdong Medical Laboratory Animal Center,Foshan 528248,China)

机构地区：[1]广东省医学实验动物中心比较医学实验室,佛山528248

出　　处：《实验动物科学》2018年第5期11-16,共6页Laboratory Animal Science

基　　金：广东省医学科研基金项目(No.B2014060);广东省科技计划项目(No.2013B060300033);广东省科技计划项目(No.2016A030303026)

摘　　要：目的比较不同的高脂饲料配方对复制混合型高脂血症大鼠模型的影响。方法 100只雄性SD大鼠随机分为基础饲料组、配方1～9饲料组,10只/组,基础饲料组大鼠每天给予基础饲料喂养,配方1～9饲料组大鼠每天给予相应饲料配方喂养,连续4周。于第2周末、第4周末检测各组大鼠血清TC、甘油三酯(TG)、高密度脂蛋白(HDLC)、低密度脂蛋白(LDLC)、血糖(GLU),于试验结束,测定肝脏系数和脂肪系数,并观察肝脏组织病理改变情况。结果与基础饲料组大鼠比较,各配方饲料组均能降低大鼠平均日摄食量,升高大鼠血清TC、LDLC含量,增大肝脏系数,加重肝细胞脂肪变性程度,具有统计学差异(P<0.05),未见对血清GLU含量有影响;各配方饲料组间比较,配方1饲料组可以明显提高血清TG含量水平(P<0.05),配方4饲料组可以明显提升血清TC、LDLC含量水平及增大肝脏系数(P<0.05),配方7饲料组引起大鼠平均日摄食量减少幅度最少(P<0.05)。结论 9个配方饲料均能复制混合型高脂血症大鼠模型,其中,配方1更有利于提高血清TG含量,配方4更有利于提高血清TC、LDLC含量,引起肝脏病变更明显,配方7最适合大鼠口感,对大鼠体质量影响最小。Objective To observe the influence of combined hyperlipidemia rat model induced by different kinds of experimental diets. Method One hundred male SD rats were randomly divided into ten groups： basic diet group, and from one to nine experimental diet group, ten rats in each group, respectively. The rats of basic diet group were treated with basic diet, while the others were treated with corresponding experimental diet for 4 weeks. Then the levels of TC, TG, HDLC, LDLC, GLU were examined after treated for 2 and 4 weeks, and the ratio of liver weight and body weight, the ratio of fat weight and body weight, and histopathology changes of the liver were examined after treated for 4 weeks. Result Compared to the basic diet group, the experimental diet groups could decrease the average daily food intake, increase the level of TC, LDLC, the ratio of liver weight and body weight, and the degree of hepatic steatosis（P〈0.05）, but did not increase the level of GLU.Comparison among the experimental diet groups, the first experimental diet group could increase the level of TG（P〈0.05）, the fourth experimental diet group could increase the level of TC, LDLC, the ratio of liver weight and body weight（P〈0.05）, the seventh experimental diet group could Less affect the average daily food intake（P〈0.05）. Conclusion The nine kinds of experimental diets could induce combined hyperlipidemia rat model, the first experimental diet group could more advantageous to increase the level of TG, the fourth experimental diet group could more advantageous to increase the level of TC, LDLC and the hepatic lesions, the seventh experimental diet group were fit for rat, and less influence on weight.

关 键 词：饲料 高脂血症 动物模型 

分 类 号：Q95-33[生物学—动物学]