＂靳三针＂疗法对额叶损伤大鼠执行功能和空间学习记忆能力的影响及其机制研究  

Effect of Jin＇s 3-needling therapy on executive function and spatial learning and memory abilities of frontal lobe injury rats

作　　者：田亮[1] 程瑞动[1] 张劼 孙鹏[1] 周亮[1] 张利[1] 李厥宝[1] 叶祥明[1] Tian Liang;Cheng Ruidong;Zhang Jie;Sun Peng;Zhou Liang;Zhang Li;Li Juebao;Ye Xiangming(Department of Rehabilitation,Zhejiang Provincial People＇s Hospital（People＇s Hospital of Hangzhou Medical College）,Hangzhou 310014,China)

机构地区：[1]浙江省人民医院(杭州医学院附属人民医院)康复医学科,杭州310014

出　　处：《中华神经医学杂志》2018年第11期1089-1096,共8页Chinese Journal of Neuromedicine

基　　金：浙江省中医药科技计划项目（2015ZB005）;浙江省医药卫生科技计划项目（2015KYB023）

摘　　要：目的探讨＂靳三针＂疗法对额叶损伤大鼠执行功能和空间学习记忆能力的影响及其机制。方法32只雄性SD大鼠按随机数字表法分为假手术组、＂靳三针＂治疗组、模型组和美多芭治疗组，每组8只。假手术组仅行开颅去骨瓣手术，不对其额叶进行撞击；模型组、＂靳三针＂治疗组和美多芭治疗组使用eCCI-6.3装置制备额叶损伤模型，其中＂靳三针＂治疗组使用＂靳三针＂疗法进行治疗，美多芭治疗组给予美多芭混悬液2 mL灌胃治疗，1次/d，持续4周。于造模后第4周末，应用GO/NO GO测试和Morris水迷宫实验评估大鼠行为学能力，额叶组织取材后应用TUNEL法检测神经细胞凋亡情况，应用免疫组化染色检测多巴胺受体D1（DRD1）表达，应用高效液相色谱分析法检测多巴胺含量。结果＂靳三针＂治疗组GO/NO GO测试的正确率明显高于美多芭治疗组，美多芭治疗组明显高于模型组，差异均有统计学意义（P〈0.05）。定位航行实验第3、4天时，美多芭治疗组和＂靳三针＂治疗组的逃避潜伏期较模型组均明显下降，＂靳三针＂治疗组较美多芭治疗组均明显下降，差异均有统计学意义（P〈0.05）。空间探索实验中，美多芭治疗组和＂靳三针＂治疗组的穿越原平台区域次数较模型组均明显增多，差异均有统计学意义（P〈0.05）。与模型组相比，美多芭治疗组和＂靳三针＂治疗组额叶组织中神经细胞凋亡率均明显减少、DRD1表达及多巴胺含量均明显增加，且＂靳三针＂治疗组神经细胞凋亡率亦较美多芭治疗组明显减少、DRD1表达及多巴胺含量亦较美多芭治疗组明显增加，差异均有统计学意义（P〈0.05）。结论＂靳三针＂疗法能够有效改善额叶损伤大鼠的执行功能和空间学习记忆能力，其机制与＂靳三针＂疗法减少额叶组织神经细胞凋亡、增加多巴胺含量及DRD1表达有关。ObjectiveTo investigate the effect of Jin＇s 3-needling therapy on executive function and spatial learning and memory abilities and their mechanism in frontal lobe injury rats.MethodsThirty-two male Sprague-Dawley rats were randomly divided into sham-operated group, Jin＇s 3-needling group, model group and medoba treatment group （n=8）. The rats in the sham-operated group only underwent craniotomy to remove the bone flap and no impact was performed on the frontal lobe; the frontal lobe injury models of model group, Jin＇s 3-needling group and medoba treatment group were established by eCCI-6.3 device; rats in the Jin＇s 3-needling group were treated with Jin＇s 3-needling therapy, and rats in the madopa treatment group were given 2 mL of madopa suspension by perfusion once daily. The behavior of rats in each group was evaluated by GO/NO GO task and Morris water maze test, and apoptosis of cells was detected by TUNEL, dopamine receptor D1 （DRD1） expression was determined by immunohistochemistry, and the content of dopamine in frontal tissues was analyzed by high performance liquid chromatography.ResultsThe accuracy rate of GO/NO GO task in Jin＇s 3 needling group was significantly higher than that in madopa treatment group （P〈0.05）; and that in madopa treatment group was significantly higher than that in model group （P〈0.05）. On the 3rd and 4th d of place navigation test, the escape latency in the Jin＇s 3 needling group and madopa treatment group was significantly decreased as compared with that in the model group （P〈0.05）; the escape latency in the Jin＇s 3 needling group was significantly decreased as compared with that in the madopa treatment group （P〈 0.05）. In spatial probe test, the number of times of crossing the platform in the Jin＇s 3 needling group and madopa treatment group was significantly larger as compared with that in the model group （P〈0.05）. As compared with those in the model group, the number of apoptotic neurons in the frontal lobes was significa

关 键 词：“靳三针”疗法 额叶损伤 执行功能 空间学习记忆能力 细胞凋亡 多 巴胺 多巴胺受体D1 

分 类 号：R651.15[医药卫生—外科学]