小蛋白质富集法鉴定酿酒酵母“漏检蛋白”  

Identification of missing proteins in Saccharomyces cerevisiae by small protein-based enrichment

作　　者：何崔同 张瑶 姜颖[1,2] 徐平 Cuitong He;Yao Zhang;Ying Jiang;and Ping Xu(Anhui Medical University,Hefei 230032,Anhui,China;Beijing Institute of Radiation Medicine,State Key Laboratory of Proteomics,National Center for Protein Sciences（Beijing）,Beijing Proteome Research Center,Beijing 102206,China;School of Life Sciences,Sun Yat-sen University,Guangzhou 510275,Guangdong,China)

机构地区：[1]安徽医科大学,安徽合肥230032 [2]军事科学院军事医学研究院辐射医学研究所、蛋白质组学国家重点实验室,国家蛋白质科学中心(北京)北京蛋白质组研究中心,北京102206 [3]中山大学生命科学学院,广东广州510275

出　　处：《生物工程学报》2018年第11期1860-1869,共10页Chinese Journal of Biotechnology

基　　金：国家重大研发计划精准医学重大专项(No.2017YFC0906600); 国家自然科学基金(No.31670834); 北京市百名领军人才计划(No.Z161100004916024)资助

摘　　要：小蛋白质(Smallproteins,SPs)是由小开放阅读框(Shortopenreadingframes,sORFs)编码长度小于100个氨基酸的多肽。研究发现小蛋白质参与了基因表达调控、细胞信号转导和代谢等重要生物学过程。然而,生命体中大多数的已注释小蛋白质尚缺少蛋白水平存在的实验证据,被称为漏检蛋白(Missing proteins,MPs)。小蛋白质的高效鉴定是其功能研究的前提,也有助于挖掘"漏检蛋白"。文中采用小蛋白质富集策略鉴定到72个酵母小蛋白质,验证9个"漏检蛋白",发现低分子量、高疏水性、膜结合、弱密码子使用偏性及不稳定性是蛋白漏检的主要原因,对进一步的技术优化具有指导意义。Small proteins（SPs） are defined as peptides of 100 amino acids or less encoded by short open reading frames（sORFs）. SPs participate in a wide range of functions in cells, including gene regulating, cell signaling and metabolism.However, most annotated SPs in all living organisms are currently lacking expression evidence at the protein level and regarded as missing proteins（MPs）. High efficient SPs identification is the prerequisite for their functional study and contribution to MPs searching. In this study, we identified 72 SPs and successfully validated 9 MPs from Saccharomyces cerevisiae based on SPs enrichment strategy. In-depth analysis showed that the missing factors of MPs were low molecular weight, low abundant, hydrophobicity, lower codon usage bias and unstable. The small protein-based enrichment can be used as MPs searching strategy, which might provide the foundation for their further function research.

关 键 词：蛋白质组学 小蛋白质 富集 漏检蛋白 

分 类 号：Q936[生物学—微生物学]