一种大豆苗期嫁接方法的建立  

Establishment of a Grafting Process at Seedling Stage of Soybean

作　　者：付星 高植 李欣欣 廖红 FU Xing;GAO Zhi;LI Xin-xin;LIAO Hong(College of Resources and Environment/Root Biology Center,Fujian Agriculture and Forestry University,Fuzhou 350002,China)

机构地区：[1]福建农林大学资源与环境学院/根系生物学研究中心,福建福州350002

出　　处：《大豆科学》2018年第6期891-895,共5页Soybean Science

基　　金：国家自然科学基金青年科学基金(31601814);中国博士后科学基金第九批特别资助项目(2016T90592)

摘　　要：为建立大豆苗期自体嫁接高成苗率的嫁接体系,本研究以Williams 82为材料,对不同生长期的幼苗进行嫁接试验,并比较不同时间的避光缓苗处理对嫁接苗成活率的影响。结果表明,利用萌发4,7和10 d的幼苗,均能进行嫁接试验。其中,生长4 d的幼苗结合3 d的避光缓苗处理,成活率高达83%。由于砧木未保留子叶,此生长期的嫁接有利于开展大豆根、冠关系的研究。当砧木保留子叶时,即利用萌发7和10 d的幼苗进行嫁接并分别避光缓苗处理3和1 d,嫁接苗均能100%成活,是一种较优的大豆嫁接体系。本研究不仅丰富和优化了大豆嫁接技术,也为大豆根-冠生理与分子机制的研究提供了技术支撑。In order to establish the grafting system of soybean seedling stage with high survival rate of self-grafting, the soy- bean variety Williams 82 was used as the material for grafting experiment at different growth stages, and the survival rate of grafting was compared as affected by different darkness treatments for recovering, The results showed that seedlings at 4,7 and 10 d after germination all can be utilized for grafting, And the survival rate was as high as 83% when the plant grown at 4 d combined with 3 d of darkness. Due to no cotyledons in the rootstock, the grafting process could contribute to study the rela- tionship between roots and shoots in soybean. When the rootstocks with cotyledons, 7 and 10 d seedlings after germination were used for grafting and separately grown under the conditions of 3 and 1 d in darkness for recovering, all grafting plants could be 100% survived, suggesed that it was a better system for soybean grafting, This study could not only enrich and opti- mize soybean grafting technique, but also provide technical support for the research on the physiological and molecular mecha- nism of the relationship between roots and shoots in soybean.

关 键 词：大豆 嫁接 方法 成活率 

分 类 号：S565.1[农业科学—作物学]