小叶三点金不同萃取相对尖吻蝮蛇毒主要酶类的抑制作用  

Inhibitory Effects of Different Extractions of Desmodium microphyllumon Activities of Major Enzymes from Deinagkistrodon acutus Venom

作　　者：熊艳 李恒[2] 余晓东[1] 龙敏 陈春妮 向柏林 朱晓艳 和七一[1] XIONG Yan;LI Heng;YU Xiaodong;LONG Min;CHEN Chunni;XIANG Bolin;ZHU Xiaoyan;HE Qiyi(Chongqing Engineering Research Center of Bioactive Substance,Ministry of Education Engineering Research Center of Active Substance and Biotechnology,College of Life Sciences,Chongqing Normal University,Chongqing 401331;Institute of Forensic Science,Criminal Police Crops of Chongqing Public Security Bureau,Chongqing 400021,China)

机构地区：[1]重庆师范大学生命科学学院教育部活性物质生物技术工程研究中心重庆市生物活性物质工程研究中心,重庆401331 [2]重庆市公安局物证鉴定中心,重庆400021

出　　处：《重庆师范大学学报（自然科学版）》2018年第6期105-111,共7页Journal of Chongqing Normal University:Natural Science

基　　金：重庆市社会民生科技创新专项(No.cstc2015shmszx120052);重庆市自然科学基金(No.cstc2016jcyjA0224)

摘　　要：【目的】考察小叶三点金(Desmodium microphyllum)乙醇提取物不同萃取相对尖吻蝮蛇(Deinagkistrodon acutus)毒主要酶类的抑制作用。【方法】采用不同极性溶剂对小叶三点金乙醇提取物进行萃取,检测各萃取相对尖吻蝮蛇毒中蛋白水解酶、磷脂酶A2、透明质酸酶和类凝血酶的抑制作用,再结合气相色谱-质谱法(GC-MS)对抑制作用最强的萃取相进行组分分析。【结果】乙酸乙酯萃取相能够明显抑制尖吻蝮蛇毒中的蛋白水解酶、磷脂酶A2和透明质酸酶和类凝血酶的活性,经GC-MS分析检测出26种成分,其中正十六烷酸、十八碳烯酸、豆甾醇和谷甾醇具有抑制蛇毒活性的作用。【结论】小叶三点金醇提物乙酸乙酯萃取相对尖吻蝮蛇毒中主要酶类具有明显抑制作用,能够作为有效部位用于抗蛇毒功效组分的筛选研究。[Purposes]To explore inhibitory effects of different extraction portions of Desmodium microphyllum ethanol extracts on activities of major enzymes fromDeinagkistrodon acutus venom.[Methods]The 75%ethanol extracts obtained fromD.microphyllum were futher fractionated by solvents with increasing polarity into five portions including petroleum ether extracts,trichloromethane extracts,ethyl acetate extracts,butyl alcohol extracts,and water extracts.The inhibitory effects of all extracts on proteolytic enzymes,phospholipase A2,hyaluronidase,and thrombin-like enzyme from D.acutus venom were detected,and the extracts with the strongest inhibitory effect was analyzed using gas chromatography-mass spectrometry（GC-MS）.[Findings]The ethyl acetate extracts significantly inhibited the activities of proteolytic enzyme,phospholipase A2,hyaluronidase,and thrombin-like enzyme from D.acutus venom.Based on the analysis of GC-MS,26 components were found in the extracts.Among which,several components were associated with inhibitory activity against snake venom,inculding n-hexadecanoic acid,vaccenic acid,sitosterol,and stigmasterol.[Conclusions]The ethyl acetate portion of ethanol extracts from D.microphyllum significantly inhibited major enzymes from D.acutus venom,and it can be used as an effective source for screening of anti-sanke venom components.

关 键 词：小叶三点金 尖吻蝮蛇毒 萃取 酶类 气相色谱-质谱法 

分 类 号：Q599[生物学—生物化学]