治疗用卡介苗对MB49膀胱肿瘤细胞增殖的影响  

Effect of BCG for therapeutic use on proliferation of bladder tumor MB49 cells

作　　者：唐溯 潘海龙[2] 潘科锦[2] 孟丽 林苹 曾献武 TANG Su;PAN Hai-long;PAN Ke-jin;MENG Li;LIN Ping;ZENG Xian-wu(State Key Laboratory of Biotherapy,Siehuan University,Chengdu 610041,Siehuan Province,China)

机构地区：[1]四川大学生物治疗国家重点实验室,四川成都610041 [2]成都生物制品研究所有限责任公司,四川成都610023 [3]中国生物技术股份有限公司,北京100029

出　　处：《中国生物制品学杂志》2018年第11期1230-1232,1236,共4页Chinese Journal of Biologicals

摘　　要：目的探讨治疗用卡介苗(简称BCGt)对MB49膀胱肿瘤细胞(简称MB49细胞)增殖的影响。方法体外培养MB49细胞,每天观察并采用CCK-8法检测细胞生长情况,绘制细胞生长曲线。取对数生长期的MB49细胞与不同浓度(20、15、10、8、6、3、2. 5、2、1. 5、1 mg/mL)的BCGt作用72 h,CCK-8法检测细胞增殖能力,计算细胞抑制率。同时采用ELISA法测定细胞培养液中IL-6、IL-8及IL-15的浓度。结果 MB49细胞于第1～4天处于对数生长期,细胞增殖速度较快,细胞形态正常。BCGt可有效抑制MB49细胞的增殖,且呈剂量依赖性,IC_(50)为3. 650 mg/mL。经BCGt作用后细胞培养液中IL-6和IL-15浓度明显增高(P <0. 05),IL-8浓度未发生明显变化(P> 0. 05)。结论BCGt可抑制MB49细胞的增殖,可能是通过提高IL-6和IL-15的水平,促进细胞免疫应答,从而发挥抗肿瘤作用的。Objective To evaluate the effect of BCG for therapeutic use（BCGt）on proliferation of bladder tumor MB49 cells. Methods MB49 cells were cultured in vitro and determined for growth by CCK-8 method daily,based on which a cell growth curve was plotted. The MB49 cells in logarithmic growth phase were treated with BCGt at various concentrations（20,15,10,8,6,3,2. 5,2,1. 5 and 1 mg/mL）for 72 h and determined by CCK-8 method,based on which the inhibitory rate of cell growth was calculated. Meanwhile,the concentrations opf IL-6,IL-8 and IL-15 in cell culture medium were determined by ELISA. Results The MB49 cells were in logarithmic growth phase within 1 ～ 4 d after culture,which grew rapidly in normal shape. BCGt inhibited the proliferation of MB49 cells effectively in a dosedependent manner,of which the IC_（50） was 3. 650 mg/mL. The concentrations of IL-6 and IL-15 in medium of cells after treatment with BCGt increased significantly（P〈0. 05）,while that of IL-8 showed no significant change（P〉0. 05）.Conclusion BCGt inhibited the proliferation of MB49 cells by a possible mechanism of increasing the IL-6 and IL-15 levels and promoting cellular immune response.

关 键 词：治疗用卡介苗 MB49膀胱肿瘤细胞 细胞增殖 CCK-8 

分 类 号：R378.911[医药卫生—病原生物学] R737.14[医药卫生—基础医学]