不同繁殖状态下绵羊繁殖相关组织中CHGA基因的表达分析  

Expression analysis of CHGA gene in reproductive related tissues of sheep(Ovis aries) during different reproductive stages

作　　者：李春艳 狄冉[1] 张彦[2] 任春环[2] 张子军[2] 田志龙 刘秋月[1] 王翔宇[1] 胡文萍[1] 张效生[4] 张金龙[4] 储明星[1] LI Chunyan;DI Ran;ZHANG Yan;REN Chunhuan;ZHANG Zijun;TIAN Zhilong;LIU Qiuyue;WANG Xiangyu;HU Wenping;ZHANG Xiaosheng;ZHANG Jinlong;CHU Mingxing(Key Laboratory of Animal Genetics,Breeding and Reproduction of Ministry of Agriculture,Institute of Animal Science,Chinese Academy of Agricultural Sciences,Beijing 100193;School of Animal Science and Technology,Anhui Agricultural University,Hefei 230036;Tianjin Institute of Animal Sciences,Tianjin 300381;College of Animal Science and Technology,Henan University of Science and Technology,Luoyang 471003)

机构地区：[1]中国农业科学院北京畜牧兽医研究所/农业部动物遗传育种与繁殖重点实验室,北京100193 [2]安徽农业大学动物科技学院,合肥230036 [3]河南科技大学动物科技学院,洛阳471003 [4]天津市畜牧兽医研究所,天津300381

出　　处：《安徽农业大学学报》2018年第5期848-852,共5页Journal of Anhui Agricultural University

基　　金：国家自然科学基金项目(31572371);国家转基因科技重大专项(2016ZX08009-003-006和2016ZX08010-005-003);中国博士后科学基金面上项目(2016M591308);国家肉羊产业技术体系专项(CARS-38);中央级公益性科研院所基本科研业务费专项(Y2017JC24,2017ywf-zd-13);中国农业科学院科技创新工程(ASTIP-IAS13,CAAS-XTCX2016010-01-03,CAAS-XTCX2016010-03-03,CAAS-XTCX2016011-02-02);内蒙古自治区科技重大专项;农业科研杰出人才及其创新团队项目;国家万人计划科技创新领军人才项目共同资助

摘　　要：为探究绵羊繁殖相关组织中CHGA基因在不同繁殖状态下的表达差异,选取不同光照条件下的成年苏尼特母羊(季节性发情品种)及不同繁殖时期的小尾寒羊母羊(常年发情品种)的下丘脑等10种组织,利用q PCR技术分析不同繁殖状态下各组织中CHGA基因的相对表达量。结果表明,CHGA基因在2个绵羊品种的不同组织中广泛表达且脑部组织中该基因的表达量高于其他组织;不同光照条件下苏尼特羊各组织中CHGA表达差异均不显著(P>0.05);黄体期小尾寒羊垂体中CHGA表达量显著高于卵泡期(P<0.05),子宫体中该基因的表达量极显著高于卵泡期(P<0.01)。以上结果暗示CHGA基因可能参与小尾寒羊不同繁殖时期垂体和子宫生理变化的调控。The main objective of the research was to explore the expression difference of CHGA gene in reproductive related tissues of sheep during different reproductive stages. The qPCR method was used to detect the relative expression of CHGA gene in ten tissues including hypothalamus in seasonal estrous sheep （Sunite sheep, SNT） at different photoperiods （short photoperiod and long photoperiod） and year-round estrous sheep （Small Tail Han sheep, STH） at different reproductive stages （follicular stage and luteal stage ）. The results indicated that CHGA gene was widely expressed in different tissues and its expression level in the brain tissues was higher than that in other tissues of two breeds. No significant difference of CHGA expression in ten tissues existed between different photoperiods of SNT （P〉0.05）. CHGA expression level in pituitary gland was significantly higher at luteal stage than that at follicular stage （P〈0.05）, while its expression level in uterine body was extremely higher at luteal stage than that at follicular stage in STH （P〈0.01）. The above results implied that CHGA gene may be involved in pituitary gland and uterus＇ physiological regulation between different reproductive stages in STH.

关 键 词：绵羊 嗜铬粒蛋白A(CHGA)基因 发情 繁殖时期 组织表达 

分 类 号：S826[农业科学—畜牧学]