基因组水平的寄生蜂基因家族扩张和收缩分析  

Genome-wide analysis of expansion and contraction of gene families in parasitic wasps

作　　者：郎坤[1] 毕守东[1] 李飞[2] LANG Kun;BI Shoudong;LI Fei(School of Science,Anhui Agricultural University,Hefei 230036;Institute of Insect Science,Zhejiang University,Hangzhou 310058)

机构地区：[1]安徽农业大学理学院,合肥230036 [2]浙江大学昆虫所,杭州310058

出　　处：《安徽农业大学学报》2018年第5期945-950,共6页Journal of Anhui Agricultural University

基　　金：国家自然科学基金(30871444);安徽省自然科学基金项目(11040606M71)共同资助

摘　　要：寄生蜂在生物防治中得到广泛的利用。随着测序技术的进步,很多寄生蜂的基因组被测序。深入研究寄生蜂的基因家族进化,对揭示寄生蜂克服寄主免疫反应,从寄主血淋巴中获得营养的分子机制等具有重要作用。对9个寄生蜂基因组数据开展了全基因组水平的基因家族分析,包括4个茧蜂科物种、4个小蜂总科物种和1个尾蜂。分析结果表明,同一个科的寄生蜂,在基因家族的扩张和收缩方面既存在共性也存在较大的差异。对同时在3个以上(含3个)物种中扩张或收缩的基因家族进行了进一步的分析,表明细胞运动、营养物质合成相关的基因家族在寄生蜂中发生了收缩和丢失,而代谢和遗传物质合成等相关的基因家族发生了明显的扩增。Parasitic wasps have been widely used in biological control for pest management. With the rapid development of next-generation sequencing technique, the genomes of an increasing number of parasitic wasps have been sequenced and publically reported. Studying the evolution of gene families in parasitic wasps should be great helpful for better understanding the molecular mechanisms of overcoming the immune response of insect hosts, and the strategy of obtaining nutrition from the host hemolymph. Here, we carried out gene family analysis of nine parasitic wasps, including 4 species in Braconidae, 4 species in Chalcidodae and 1 species in caudal. Genome-level analysis showed these wasps shared common features of gene expansion and contraction. However, differences in terms of gene expansion and contraction were also observed in two different subfamilies of Braco- nidae or Chalcidodae. The gene families which expand or contract in 3 or more wasp species were analyzed, which showed that the gene families related to cell movement and nutrient synthesis were contracted, and the genes related to metabolism and genetic material synthesis were expanded significantly.

关 键 词：寄生蜂 全基因组 系统发育 基因家族 扩张与收缩 

分 类 号：Q963[生物学—昆虫学] S435[农业科学—农业昆虫与害虫防治]