HPLC波长切换法测定复方石淋通片中绿原酸和夏佛塔苷的含量  

Simultaneous Determination of Chlorogenic Acid and Schaftoside in Fufang ShiLinTong Tablets by Wave-length Switching HPLC

作　　者：马杰 彭玲娜 童珂 潘震宇 李瑞莲 MA Jie;PENG Ling-na;TONG Ke;PAN Zhen-yu;Li Rui-lian(].Hunan institute for drug control,Changsha,410001,China;Hunan engineering ＆ Technology Research Center for Pharmaceutical Quality Evaluation,Changsha 410001,China)

机构地区：[1]湖南省药品检验研究院,长沙410001 [2]湖南省药品质量评价工程技术中心,长沙410001

出　　处：《海峡药学》2018年第11期58-60,共3页Strait Pharmaceutical Journal

摘　　要：目的建立同时测定复方石淋通片中绿原酸和夏佛塔苷的方法。方法采用高效液相色谱方法,色谱柱为Agilent Zorbax C_(18),流动相为乙腈和0. 1%磷酸溶液梯度洗脱;流速1. 0mL·min^(-1);检测波长0～25min为327nm,25～50min为272nm;柱温30℃。结果绿原酸进样量在0. 016～1. 009μg(r=0. 9999)范围内线性关系良好,平均加样回收率为99. 1%,RSD=1. 4%(n=6)。夏佛塔苷进样量在0. 016～1. 048μg(r=0. 9999)范围内线性关系良好,平均加样回收率为101. 3%,RSD=1. 2%(n=6)。结论所采用的方法准确、可靠,专属性及重复性良好,适用于复方石淋通片的质量控制。OBJECTIVE To establish a method for simultaneous determination of chlorogenic acid and schaflo- side in Fufang ShiLinTong tablets. METHODS HPLC was performed on the column of Agilent Zorbax C18 with mo- bile phase of acetonitrile-0, l% phosphoric acid （gradient elution） at the rate of 1.0mL ·min-1 ,the detection wavelength were 327 nm （0 - 25 min） for chlorogenic acid and 272nm （ 25 - 50min） for schaftoside, the column temperature was 30℃. RESULTS The Calibration curves were linear within the range of 0. 016 - 1. 009μg for chlorogenic acid and 0. 016 - 1. 048μg for schaftoside. All ingredients showed a good linear relationship（ r = 0. 9999 ）. The average recoveries were 99. 1% and 101.3% ,respectively. RSDs were1. 4% and 1.2% , respectively. CONCLUSION The method is accurate, reliable, specific and with good repeatability, and can be used for control the quality of the preparation more effectively.

关 键 词：复方石淋通片 绿原酸 夏佛塔苷 波长切换法 

分 类 号：R927[医药卫生—药学]