大鼠脑缺血再灌注后环氧合酶-2促进E-选择素表达增多及灯盏花素的脑保护作用  

Cyclooxygenase-2 promoting the expression of E-selectin and the cerebral protective effects of breviscapine after cerebral ischemic-reperfusion injury in rats

作　　者：杨迎春[1] 任占川[1] 陈新骥[2] YANG Ying-chun;REN Zhan-chuan;CHEN Xin-ji(Department of Anatomy;Anatomy Laboratory,Fenyang Colleage of Shanxi Medical University,Shanxi Fenyang 032200,China)

机构地区：[1]山西医科大学汾阳学院解剖学教研室 [2]山西医科大学汾阳学院解剖学实验室,山西汾阳032200

出　　处：《解剖学报》2018年第6期703-707,共5页Acta Anatomica Sinica

基　　金：山西省高校科技研究开发项目(200613049)

摘　　要：目的观察大鼠脑缺血再灌后环氧合酶-2(COX-2)对E-选择素(E-selectin)表达的影响及灯盏花素对COX-2及E-selectin的影响,探讨脑缺血再灌注损伤后, COX-2加重脑损伤的机制及灯盏花素的脑保护作用。方法 48只清洁级SD大鼠随机分为假手术组、生理盐水组、SC-58125组、灯盏花素治疗组,在缺血2 h再灌注24 h时,神经功能学评分观察大鼠神经行为的变化,2,3,5-氯化三苯四氮唑(TTC)染色观察大鼠脑梗死灶体积的变化;免疫印迹法和酶联免疫吸附测定检测COX-2及E-selectin表达的变化。结果 SC-58125组和灯盏花素治疗组神经行为学评分明显降低,脑组织梗死体积显著减小,COX-2和E-selectin的表达明显下降。结论脑缺血再灌注后,COX-2可能通过促进E-selectin的表达加重缺血区的炎症反应;灯盏花素可能通过抑制COX-2及E-selectin的表达而发挥脑保护作用。Objective To investigate the mechanisms of cyclooxygenase-2 （COX-2） aggravating cerebral injury and the protective effects of breviscapine after cerebral ischcmic-reperfusion injury, by observing the effect of COX-2 on the expression of E-selectin and the effect of breviscapine on the expression of COX-2 and E-selectin. Methods Forty-eight SD rats were randomly divided into sham group, saline group, SC-58125 group and hreviscapine treatment group, at ischemia hour 2/reperfusion hour 24. Nerve function scores were used to observe the neurobehavioral changes of rats, 2,3,5-triphenyhetrazolium chloride （TTC） staining was used to assess the changes of cerebral infarction volume. Western blotting and enzyme-linked immunosorbent assay were used to detect the expression of COX-2 and E-selectin. Results In SC-58125 group and breviscapine treatment group, nerve function scores, cerebral infarction volume and COX-2 and E-selectin were significantly decreased. Conclusion COX-2 aggravates inflammatory response of ischemia area by promoting the expression of E-selectin; breviscapine may play a protective role by inhibiting the expression of COX-2 and E-selectin.

关 键 词：大脑 缺血再灌注损伤 环氧合酶-2 E-选择素 灯盏花素 免疫印迹法 大鼠 

分 类 号：R322.8[医药卫生—人体解剖和组织胚胎学]