健脾化瘀方对肝癌HepG-2细胞生理活性的影响  

Effects of Spleen-invigorating Stasis-eliminating Prescription on Physiological Activity of HepG-2 Cells in Liver Cancer

作　　者：林泳欣 王昌俊[2] LIN Yongxin;WANG Changjun(Guangzhou University of Chinese Medicine,Guangzhou 510000,China;Guangdong Central Hospital /Guangdong Academy of Medical Sciences)

机构地区：[1]广州中医药大学,广东广州510000 [2]广东省人民医院/广东省医学科学院

出　　处：《西部中医药》2018年第11期11-13,共3页Western Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金(编号81373582;81774261);广东省中医药局科研项目(编号20181008)

摘　　要：目的:探讨健脾化瘀方对人肝癌HepG-2细胞侵袭、转移及凋亡的影响。方法:选择人肝癌HepG-2细胞作为实验细胞,以CCK-8、细胞迁移实验(Transwel l)、流式细胞术分别检测健脾化瘀方对HepG-2细胞的增殖、转移能力以及凋亡的干预作用。结果:药物作用HepG-2细胞48小时的半抑制浓度(IC50)为12. 15,72小时的I C50为9. 75;药物作用24小时后,Hep G-2细胞迁移率为(42. 11±8. 49)%;总凋亡率为(20. 23±1. 15)%。结论:健脾化瘀方对Hep G-2细胞有抑制作用,尤以作用48小时的I C50更显著;且对Hep G-2细胞有抑制转移和促进凋亡的效应。Objective： To discuss the effects of spleen-invigorating stasis-eliminating prescription on theinvasion, metasis and apoptosis of human liver cancer HepG-2 cells. Methods： Human liver cancer HepG-2 cellswere chosen as the experimental cells, the intervention effects of spleen-invigorating stasis-eliminating prescriptionon the proliferation, metasis of HepG-2 cells were detected by CCK-8, Transwell and flow cytometry. Results： IC50responses to HepG-2 cells after the medicines acted on for 48 hours was 12.15, IC50 for 72 hours was 9.75; HepG-2cell mobility was （42.11 ±8.49）%; total apoptosis rate was （20.23 ±1.15）%. Conclusion： Spleen-invigorating stasis-eliminating prescription possess the inhibitory effects to HepG-2 cells, especially, IC50 after acted on for 48 hours themost significant; and it has the effects of inhibiting metastasis and promoting apoptosis on HepG-2 cells.

关 键 词：肝癌 侵袭 转移 凋亡 健脾化瘀方 

分 类 号：R256.4[医药卫生—中医内科学]