灸神阙、针刺组穴对抑郁型大鼠行为学影响  

Effect of Moxibustion on Shenque and Acupuncture at Acupoints on Behavior of Depressed rats

作　　者：韩兴军 郑雅峰 王璇 HAN Xingjun;ZHENG Yafeng;WANG Xuan(The Second Affiliated Hospital of Shandong University of Traditional Chinese Medicine,Jinan 250001,Shandong,China)

机构地区：[1]山东中医药大学第二附属医院

出　　处：《辽宁中医药大学学报》2018年第11期135-139,共5页Journal of Liaoning University of Traditional Chinese Medicine

基　　金：山东省自然科学基金项目(ZR2014HL099)

摘　　要：目的：观察灸神阙、针刺、氟西汀灌胃干预抑郁型大鼠的疗效差异。方法：选择Open-Field法评分相近的30只SD大鼠,随机分为5组,分别为灸神阙组（艾灸组）、针刺组、氟西汀组（西药组）、抑郁空白组（模型组）、正常对照组（对照组）,每组6只,采用孤养结合慢性应激的方法造模。实验第21天,进入干预阶段。对照组：常规饲养;模型组：孤笼,常规饲养;艾灸组：选取神阙穴,以温开水调面粉成圈状,其内径与约1 cm,再将备好的药末均匀地填满脐部,将艾炷置于药末上,施灸,连续施灸2～3壮,约20 min,每3 d 1次,1周为1个疗程,3个疗程后观察疗效;针刺组：穴位选取百会、印堂、劳宫、神门以及太冲穴,应用30号1.0寸针灸针,百会穴、印堂向前斜刺,余穴直刺,留针20 min,1次/d,连续21 d。西药组进行灌胃给药,用蒸馏水配成1.8 mg/kg,按10 mL/kg给药,1次/d,连续21 d。通过记录抑郁模型大鼠体质量变化、糖水消耗实验及旷场实验等行为学检测指标数据,观察模型大鼠抑郁症状的持续情况,明确抑郁症模型的稳定性及灸神阙、针刺组穴对抑郁型大鼠行为学的影响。结果：在造模21 d,模型组大鼠的行为学表现、体质量变化、糖水消耗率与对照组相比较均明显下降（P〈0.05）。经过3周的不同干预,第42天,针刺组、艾灸组、西药组大鼠的体质量变化、糖水消耗实验及旷场实验各项指标均明显优于模型组大鼠（P〈0.05）,艾灸组和针刺组的抗抑郁效果优于西药组（P〈0.05）,大鼠体质量方面,针刺组和艾灸组组间比较无显著性差异,P〉0.05;糖水消耗试验、Open-Field实验,艾灸组的抗抑郁效果优于针刺组（P〈0.05）。结论：脐疗、针灸疗法能改善抑郁症大鼠抑郁状态,在治疗方面显示了独特的优势,其安全性高,副作用小,同时患者容易接受,值得临床上广泛推广。Objective：To observe the effects of moxibustion Shenque,acupuncture,fluoxetine gavage on depressive rats. Methods：Thirty Sprague-Dawley rats with similar score of Open-Field method were randomly divided into 5 groups：moxibustion Shenque group（moxibustion group）,acupuncture group,fluoxetine group（Western medicine group）,depression group（model group）,normal control group（control group）,6 cases in each group,modeling by solitary feeding combined with chronic stress. Therapy was started on the 21 st day,normal group,conventional feeding;model group,lone cage,routine feeding;moxibustion group selected Shenque acupuncture to warm open the water into the flour ring shape,its diameter and about 1 cm,and then prepare the medicine evenly fill the umbilical end,the moxa cone on the end of medicine,moxibustion,continuous moxibustion 2-3 Zhuang,about 20 min,once every three days,a week for a course of treatment,after 3 courses of observation efficacy;Acupuncture group selected Baihui,Yintang,Laogong,Shenmen and Taichong points. Rats in acupuncture group were acupunctured with 30-sized 1.0 inch needle at Baihui and Yintang sloping forwardly in depth of 2 mm. The rest of the points in depth of 1 mm. Needle was maintained for 20 min,once a day for 21 days. Western medicine group for intragastric administration,with distilled water dubbed 1.8 mg/kg,according to 10 mL/kg dosing,once a day,for 21 days. By recording the changes of body weight,glucose consumption and open field in depression model rats,the behavioral indexes were used to detect the persistence of depressive symptoms and the stability of depression model and moxibustion Shenque,acupuncture points on the behavior of depression rats. Results：On the 21 st day of modeling,the behavior,body weight and the rate of water consumption of the model group were significantly decreased compared with the control group（P〈0.05）. After three weeks of different interventions,on the 42 nd day,the acupuncture group,moxibustion group and Western medicine grou

关 键 词：抑郁症 艾灸 针刺 慢性应激 氟西汀 行为学 

分 类 号：R245[医药卫生—针灸推拿学]