硅酸镁纳米空心球的制备及对蛋白质负载和释放性能的研究  

Synthesis of uniform magnesium silicate hollow spheres and investigation of protein loading and release performance

作　　者：方娴韵 俞淑佳 陈旖婷 谢亦秋 蒋淑琴 FANG Xianyun;YU Shujia;CHEN Yiting;XIE Yiqiu;JIANG Shuqin(School of Public Health,Capital Medical University,Beijing 100069;School of Basic Medical Sciences,Capital Medical University,Beijing 100069)

机构地区：[1]首都医科大学公共卫生学院,北京100069 [2]首都医科大学基础医学院,北京100069

出　　处：《北京生物医学工程》2018年第6期589-596,共8页Beijing Biomedical Engineering

基　　金：国家自然科学基金青年项目(21601129)资助

摘　　要：目的空心结构的硅酸镁纳米材料因其较大的比表面积和丰富的介孔结构在生物医学领域逐渐受到重视。本文采用模板法制备硅酸镁纳米空心球,并分析其生物相容性、降解性能及对蛋白质的负载和释放性能,以研究其在蛋白质药物递送方面的潜在应用。方法以纳米二氧化硅为模板,采用牺牲模板法制备了硅酸镁纳米空心球。采用透射电镜、扫描电镜、动态光散射粒度仪、X射线衍射仪等对制备材料的形貌和结构进行了详细表征。采用CCK-8实验检测制备材料的细胞毒性。此外,通过紫外-可见光谱仪分析了制备材料对蛋白质的吸附和释放行为,并分别采用Langmuir,Freundlich和Temkin三种等温吸附模型对蛋白质的具体吸附行为进行了详细分析。结果理化性质表征显示制备材料形貌规整,大小较为均一,比表面积较大(412. 35 m2/g)。细胞实验中,当硅酸镁纳米空心球在250μg/m L的高浓度条件下,细胞存活率也在90%左右。降解实验表明,制备材料在酸性条件下能达到约80%降解。蛋白质负载与释放实验中,当蛋白质浓度为3. 2 mg/m L时,负载量达到225 mg/g;负载的蛋白质在中性和酸性条件下均可以释放出来,尤其在酸性条件下累计释放量更多。吸附模型分析结果表明,蛋白质的吸附符合Freundlich等温线模型。结论硅酸镁纳米空心球具有良好的生物相容性和降解性能,并且对蛋白质具有很好的负载和pH响应的释放行为,作为载体在生物医学领域如蛋白质等生物分子的递送方面具有广阔的应用前景。Objective Magnesium silicate hollow spheres （MgSiO3, denoted as NPs） are getting moreattentions in biomedical applications due to its high surface area and mesoporous structures. Therefore, to studypotential applications of NPs as protein drug delivery, we synthesize NPs by template method and analyze theircytocompatibility, degradation, adsorption and release properties of proteins. Methods We used nano SiO2 astemplate to synthesis NPs, and TEM, SEM, DLS and XRD techniques were applied to characterize the morphologies and structures of obtained NPs. CCK-8 experiments were used to test cytotoxicity of synthesizedNPs. Moreover, we studied the protein absorption andrelease of NPs by UV-Vis spectrophotometry, andLangmuir, Freundlich and Temkin isothermal adsorption models were applied to analysis the adsorption behavior. Results Characterization of synthesized NPs exhibiteduniform hollow spheres and high surface area （412. 35 m^2/ g）. In cell experiments, cell viability were about90% even at high concentration of NPs （250 μg / mL）. In degradation experiments, synthesized NPs degraded80% in acid environments. In protein adsorption and release experiments, when the protein concentration was3. 2 mg / mL, the loading amount of protein could be 225 mg / g. In addition, loading protein could be releasedin neutral and acidic conditions, especially in acidic conditions. In adsorption isotherm model experiments,Freundlich adsorption isotherm model fitted well with BSA adsorption data. Conclusions Therefore, thesynthesized MgSiO3 hollow spheres exhibit good biocompatibility and degradation property, high protein loadingcapacity, and release of protein as pH.controllable. So, these NPs are more promising for applications inbiomedical fields, for example, protein adsorption and delivery.

关 键 词：硅酸镁 纳米空心球 生物相容性 降解性能 吸附 

分 类 号：R318.04[医药卫生—生物医学工程] O614.22[医药卫生—基础医学]