紫外-可见分光光度法测定那如-3药材提取物中乌头碱含量  

UV-Vis Spectrophotometry Determination of Aconitine in Naru-3 Extracts

作　　者：李君[1] 张微[1] 赛那 杨敏 顾艳丽 Li Jun;Zhang Wei;Sai Na;Yang Min;Gu Yanli(The Center For New Drug Safety Evaluation And Research Of Inner Mongolia Medical University,Inner Mongolia Hohhot 010110;College Of Pharmacy,Inner Mongolia Medical University,Inner Mongolia Hohhot 010110;Ulan Qab Central Hospital,Inner Mongolia Ulan Qab 012099)

机构地区：[1]内蒙古医科大学新药安全评价中心,呼和浩特010110 [2]内蒙古医科大学药学院,呼和浩特010110 [3]乌兰察布市中心医院,乌兰察布012099

出　　处：《北方药学》2018年第12期1-2,共2页Journal of North Pharmacy

基　　金：内蒙古自治区科技创新引导项目(1639002);国家自然科学基金委地区项目(81673695)

摘　　要：目的:建立检测蒙药那如-3药材提取物中乌头碱含量的方法。方法:根据酸性染料比色法原理,采用紫外-可见分光光度法测定,检测波长415nm。结果:乌头碱在3.904~27.328μg/mL范围内线性关系良好,回归方程:Y=0.0249X+0.0768,r=0.9999,平均回收率99.87%。结论:该方法简便、灵敏,重复性良好,可用于蒙药那如-3药材提取物中乌头碱的含量测定。Objective：To establish a determination method for content of Aconitine in the Mongolian Medicine Naru-3 extract. Methods：The content of Aconitine was determined at wavelength of 415 nm by means of Acid dye colorimetry. Results：Aconitine had a good linear relationship in the range of 3.904~27.328μg/mL and the regression equation was Y=0.0249 X＋0.0768,r=0.9999,the average recovery of Aconitine was 99.87%. Conclusion：This method is simple,sensitive and good reproducibilite.It can be used for determination of Aconitine content in the Mongolian Medicine Naru-3 extract.

关 键 词：那如-3 乌头碱 含量测定 紫外-可见分光光度法 

分 类 号：R284.1[医药卫生—中药学]