松果菊苷对6-OHDA帕金森病模型大鼠海马α-Synuclein、β-actin蛋白表达影响随机平行对照研究  

Randomized Paralleled Controlled Study of the Effect of Echinacoside on the Expression of α-Synuclein Protein in Hippocampus of Rats with Parkinson's Disease

作　　者：周紫婷[1] 付贝贝[1] 覃威 陈诗雅[1] 张龙惠 蔡晶[1] ZHOU Ziting;FU Beibei;TAN Wei;CHEN Shiya;ZHANG Longhui;CAI Jing(Department of biostatisties,School of Integrated Traditional Chinese and Western medicine,Fujian University of Traditional Chinese Medicine Grade 2013 clinical medicine,Fuzhou 350122)

机构地区：[1]福建中医药大学中西医结合学院,福州350122

出　　处：《实用中医内科杂志》2018年第9期57-61,共5页Journal of Practical Traditional Chinese Internal Medicine

基　　金：福建省科技厅引导性项目(2017Y0053);福建省中医药科研项目(2017FJZYLC501)~~

摘　　要：[目的]观察松果菊苷对6-OHDA帕金森病模型大鼠海马α-Synuclein、β-actin蛋白表达影响。[方法]使用随机平行对照方法,将72只SPF级SD雄性大鼠适应性饲养14d,按随机数字表法分为6组,空白对照组、假手术组、模型组、松果菊苷(低、中、高剂量)组,12只/组。各组大鼠腹腔注射10%水合氯醛(30mg/kg)麻醉,固定于颅脑定位仪,消毒,沿颅顶正中线切开皮肤,钝性分离颅骨外膜,暴露大鼠颅骨前囟门;以前囟为坐标原点(大鼠脑定位立体图谱),确定前脑内侧束(MFB)坐标,MFB:前囟后4.8mm,矢状缝右侧1.2mm,硬脑下7.8mm;颅骨钻钻孔,立体定向靶点微量注射6-OHDA(2ug/uL,溶于0.1%抗坏血酸生理盐水),注射速度1uL/min,留针15min;骨蜡封闭大鼠脑部颅骨孔,消毒缝合皮肤,复制帕金森病模型,松果菊苷(低、中、高剂量)组(8 ug)、模型组(2ug);空白对照组、假手术组(与模型组等量生理盐水)。灌胃干预:复制成功模型,松果菊苷组(低20mg/kg、中40mg/kg,高80mg/kg),余各组均等体积生理盐水,1次/d,连续10d。PAGE胶蛋白电泳;免疫印迹(Western Blot,WB)法检测α-Synuclein、β-actin蛋白。[结果]模型复制48只大鼠,麻醉过度死亡3只,模型复制不成功2只,最终纳入结果分析43只,模型组10只,松果菊苷组低、中、高剂量组各11只。α-Synuclein蛋白水平假手术组、松果菊苷各组(低中高)均低于空白对照组(P<0.01);β-actin各组间无明显差异(P>0.05);α-Synuclein/β-actin假手术组、松果菊苷低剂量组、松果菊苷中剂量组、松果菊苷高剂量组均低于空白对照组(P<0.01)。[结论]松果菊苷干预帕金森病模型大鼠,可降低海马α-Synuclein表达。[Objective] To observe the effect of echinacoside on the expression of α-Synuclein and β-actin protein in hippocampus of rats with Parkinson＇s disease. [Method] Using the randomized parallel control method, 72 SPF SD rats were randomly divided into 6 groups according to the random number table method,including the normal group, the sham group, the model group, the low dose group of echinoside, the medium dose group of echinoside and the high dose group of echinoside. Rats were intraperitoneally injected with10% chloral hydrate（30 mg/kg）. After anesthesia, the rats were immobilized on the craniocerebral locator and routinely disinfected. The skin was cut along the median line of the cranial top. The outer membrane of the skull was blunt, exposing the anterior fontanelle of the skull in rats. Referring to the stereotaxic map of the brain in rats, the coordinates of the medial bundle（MFB） of the forebrain were determined by the original origin of the fontanelle as the coordinate origin. MFB： 4.8 mm after anterior fontanelle, 1.2 mm on the right side of sagittal suture, 7.8 mm under the dura mater. The skull was drilled, and 6-OHDA（2 ug/uL, dissolved in 0.1% ascorbic acid physiological saline） was injected at a rate of 1 uL/min, and the needle was kept for 15minutes. The brain cranial foramen of rats were closed by bone wax, the skin was disinfected and sutured, and the Parkinson＇s syndrome model was replicated. The blank control group and the sham operation group（the same physiological saline as the model group）. Gavage intervention.After selecting a successful model, the drug intervention was performed,the dosage of the low dose group was 20 mg/kg, the middle dose group was 40 mg/kg, the high dose group was 80 mg/kg, the rest of the other groups were irrigated with the same volume of saline, 1 times/d, for 10 days. Detection of α-Synuclein protein. [Result] In 72 rats, 3 rats died of excessive anesthesia during the process of modeling, and 2 of them were not eliminated successfully, and 43

关 键 词：帕金森病 雄性SPF级SD大鼠 6-OHDA 动物模型 松果菊苷 高中低剂量 海马 脑定位立体图谱 靶点注射 蛋白电泳 免疫印迹(Western Blot WB) α-Synuclein蛋白 β-actin蛋白 实验研究 随机平行对照研究 

分 类 号：R285.5[医药卫生—中药学] R-332[医药卫生—中医学]