猴头菌浸膏多糖及蛋白质含量测定方法的比较  

Comparison of the methods for determination of polysaccharide and protein in Hericium erinaceus(Bull.:Fr.) extractum

作　　者：王新宇[1] 刘振华 李影丹[1] 徐涵宇 尚红梅[4] 宋慧[3] WANG Xinyu;LIU Zhenhua;LI Yingdan;XU Hanyu;SHANG Hongmei;SONG Hui(Pharmaceutical Factory of Bethune University of Medical Science,Changehun 130012,China;Department of Digestive of China-Japan Union Hospital of Jilin University,Changchun 130033,China;College of Life Science,Jilin Agricultural University,Changchun 130118,China;College of Animal Science and Technology,Jilin Agricultural University,Changchun 130118,China)

机构地区：[1]白求恩医科大学制药厂,长春130012 [2]吉林大学中日联谊医院消化内科,长春130033 [3]吉林农业大学生命科学学院,长春130118 [4]吉林农业大学动物科学技术学院,长春130118

出　　处：《黑龙江畜牧兽医》2018年第21期219-223,共5页Heilongjiang Animal Science And veterinary Medicine

基　　金：国家自然科学基金青年科学基金项目(31601972);吉林省世行贷款农产品质量安全项目(2011-Y18)

摘　　要：为了更好地控制猴头菌浸膏的质量,试验分别采用3,5-二硝基水杨酸法、蒽酮-硫酸法和苯酚-硫酸法测定猴头菌浸膏中多糖的含量,分别采用福林酚法和考马斯亮蓝法测定猴头菌浸膏中蛋白质的含量,从中筛选出适合猴头菌浸膏的多糖及蛋白质含量的测定方法。结果表明：用蒽酮-硫酸法测定多糖含量时,结果高于其他两种方法,且稳定性、精密度、加标回收率、重复性的相对标准偏差（RSD）均小于5. 00%;用福林酚法测定蛋白质含量时,结果高于考马斯亮蓝法,且稳定性、精密度、重复性及加标回收率的RSD均小于5. 00%。说明蒽酮-硫酸法操作简便、稳定可行,是最适合测定猴头菌浸膏中多糖含量的方法;福林酚法操作简便、稳定可行,是最适合测定猴头菌浸膏中蛋白质含量的方法。The aim of the present study was to better control the quality of Hericium erinaceus extractum. The contents of polysaccharides in Hericium erinaceus extractum were determined by 3,5-dinitrosalicylic acid method,anthrone-sulfuric acid method and phenol-sulfuric acid method,respectively. And the content of protein in Hericium erinaceus extractum was determined by Folin phenol method and Coomassie brilliant blue method. Then the suitable method for the determination of polysaccharides and protein in Hericium erinaceus extractum was selected. The results showed that the content of polysaccharides determined by anthrone-sulfuric acid method was higher than that by the other two methods,and the relative standard deviation（RSD） of stability,precision,repeatability and recovery rate of the method were all less than 5. 00%. The content of protein determined by Folin phenol method was higher than that by Coomassie brilliant blue method. And the RSD of stability,precision,repeatability and recovery of standard addition were all less than 5. 00%.The results indicated that the method of anthrone-sulfuric acid was simple,stable and feasible,and it was the most suitable method for the determination of polysaccharides in Hericium erinaceus extractum. The method of forint phenol was simple,stable and feasible,and it is the most suitable method for the determination of protein in Hericium erinaceus extractum.

关 键 词：猴头菌浸膏 多糖 蛋白质 苯酚-硫酸法 蒽酮-硫酸法 3 5-二硝基水杨酸法 福林酚法 考马斯亮蓝法 

分 类 号：Q539[生物学—生物化学] Q51