Eco-friendly and Cleaner Process Using Online Microwave-assisted Steam Extraction Coupled with Solid-phase Extraction for Trace Analysis of Sulfonamides in Animal Feed  

作　　者：LI Guijie LIU Chang WANG Dawei DING Lan 

机构地区：[1]College of Food Science, Jilin Agricultural University, Changchun 130118, P. R. China [2]College of Chemistry, Jilin University, Changchun 130012, P. R. China [3]Jilin Province Product Quality Supervision and Inspection Institute, Changchun 130103, P. R. China

出　　处：《Chemical Research in Chinese Universities》2018年第6期893-898,共6页高等学校化学研究（英文版）

基　　金：Supported by the National High Technology Research and Development Program of China（No.2011AA100805） and the National Natural Science Foundation of China（No.20875037）.

摘　　要：An environmentally friendly method for extracting sulfonamides（SAs） residues from animal feed was described and applied. The method used online microwave-assisted steam extraction coupled with solid phase extraction（MASE-SPE）, which was followed by the analysis using high performance liquid chromatography-mass spectro- metry（HPLC-MS/MS）. The SAs residues were extracted successively with water steam under microwave irradiation, and thus directly introduced into an SPE colunm containing cation-exchange resin. The SAs were then eluted with methanol-ammonia（90：10, volume ratio） from the SPE column and followed by HPLC-MS/IVIS. The limits of detec- tion（LODs） for the analytes ranged from 0.24 ng/g to 0.49 ng/g. The limits of quantification（LOQs） ranged from 0.82 ng/g to 1.63 ng/g. Average recoveries of SAs were 76.3%--92.1%. The developed method was a reliable and envi-ronmentally friendly alternative to previous methods with respect to time, solvent and labor consumption for the analysis of SAs in animal foodstuffs.An environmentally friendly method for extracting sulfonamides（SAs） residues from animal feed was described and applied. The method used online microwave-assisted steam extraction coupled with solid phase extraction（MASE-SPE）, which was followed by the analysis using high performance liquid chromatography-mass spectro- metry（HPLC-MS/MS）. The SAs residues were extracted successively with water steam under microwave irradiation, and thus directly introduced into an SPE colunm containing cation-exchange resin. The SAs were then eluted with methanol-ammonia（90：10, volume ratio） from the SPE column and followed by HPLC-MS/IVIS. The limits of detec- tion（LODs） for the analytes ranged from 0.24 ng/g to 0.49 ng/g. The limits of quantification（LOQs） ranged from 0.82 ng/g to 1.63 ng/g. Average recoveries of SAs were 76.3%--92.1%. The developed method was a reliable and envi-ronmentally friendly alternative to previous methods with respect to time, solvent and labor consumption for the analysis of SAs in animal foodstuffs.

关 键 词：High performance liquid chromatography-mass spectrometry Microwave-assisted steam extraction Solid-phase extraction SULFONAMIDE Animal feed 

分 类 号：TQ116.2[化学工程—无机化工] S859.84[农业科学—临床兽医学]