Graphene-Gold Nanoparticle-modified Electrochemical Sensor for Detection of Kanamycin Based on Target-induced Aptamer Displacement  

作　　者：ZHENG Jingyi FENG Rongrong HE Caimei LI Xiaoxia 

机构地区：[1]School of Chemistry and Chemical Engineering, Yah'an University, Yah'an 716000, P. R. China

出　　处：《Chemical Research in Chinese Universities》2018年第6期952-958,共7页高等学校化学研究（英文版）

基　　金：Supported by the Natural Science Foundation of Yan＇an University, China（No. YDY2018-07）.

摘　　要：A highly sensitive and selective label-free electrochemical sensor was developed for the determination of kanamycin. To improve the sensitivity of the electrochemical sensor, graphene-gold nanoparticles were prepared by a one-step electrochemical coreduction process and were modified on the surface of a glassy carbon electrode. The double-stranded DNA（ds-DNA） duplex probe was immobilized onto the graphene-gold nanoparticle-modified elec-trode. The introduction of target kanamycin induced the displacement of aptamer from the ds-DNA duplex into the solution. Methylene blue（MB） as a redox indicator monitored the current change using differential pulse voltammetry Under optimal conditions, the designed electrochemical aptasensor exhibited a wide linear range from 0. i pmol/L to 10 pmol/L with a detection limit of 0.03 pmol/L for kanamycin. The experimental strategy enabled the direct analysis of milk samples, and the results showed high sensitivity and good selectivity.A highly sensitive and selective label-free electrochemical sensor was developed for the determination of kanamycin. To improve the sensitivity of the electrochemical sensor, graphene-gold nanoparticles were prepared by a one-step electrochemical coreduction process and were modified on the surface of a glassy carbon electrode. The double-stranded DNA（ds-DNA） duplex probe was immobilized onto the graphene-gold nanoparticle-modified elec-trode. The introduction of target kanamycin induced the displacement of aptamer from the ds-DNA duplex into the solution. Methylene blue（MB） as a redox indicator monitored the current change using differential pulse voltammetry Under optimal conditions, the designed electrochemical aptasensor exhibited a wide linear range from 0. i pmol/L to 10 pmol/L with a detection limit of 0.03 pmol/L for kanamycin. The experimental strategy enabled the direct analysis of milk samples, and the results showed high sensitivity and good selectivity.

关 键 词：KANAMYCIN Electrochemical aptasensor GRAPHENE Gold nanoparticle Methylene blue 

分 类 号：Q52[生物学—生物化学] O625.463[理学—有机化学]