大穿透深度折射率传感器对活细胞药物敏感性的研究  

Study on Large Penetration Depth Refractive Index Sensor to Drug Susceptibility of Living Cells

作　　者：刘璐[1] 吴晓静[2] 孙伽略 张慧琴 杨勇[1] Liu Lu;Wu Xiaojing;Sun Jialue;Zhang Huiqin;Yang Yong(Institute of Modern Optics,Nankai University,Tianjin 300350,China;Tianjin Union Medical Center,Tianjin 300121,China)

机构地区：[1]南开大学现代光学研究所,天津300350 [2]天津市人民医院,天津300121

出　　处：《光学学报》2018年第11期14-20,共7页Acta Optica Sinica

基　　金：国家自然科学基金(11474171);天津市卫生和计划生育委员会科技基金(2015KY30)

摘　　要：针对现有表面等离子激元折射率传感器纵向探测深度小、探测范围无法覆盖整个细胞厚度的问题,提出一种大探测深度、高灵敏度的活细胞折射率实时测量方法,并利用该方法开展了药物敏感性的实验研究。基于偏振选择吸收效应,设计并搭建了全内反射条件下的石墨烯折射率传感系统,进行了不同质量分数氯化钠溶液折射率的测量,结果表明系统具有9.5×10~6 mV/RIU的灵敏度和5.5×10^(-7) RIU的分辨率;利用该系统开展了活细胞药物敏感性的实验研究,分别研究了顺铂和紫杉醇作用于Ramos细胞和Jeko-1细胞时生物演化过程中细胞折射率的实时变化规律,验证了折射率变化与其药性机理作用的一致性。Aiming at the problem that the existing surface detection depth and the detection range cannot cover the plasmon refractive index sensor has a small vertical entire thickness of cells, we propose a real-time measurement method of the living cell refractive index for its advantages： large detection depth and high sensitivity. And this method is used to carry out the experimental research on drug susceptibility. Based on the polarizationselective absorption effect, we design and build a graphene-based refractive index sensing system under the condition of total internal reflection. The refractive index with various mass fraction of sodium chloride solution is measured. The results indicate that the sensitivity and resolution of the system is 9.5 × 10^6 mV/RIU and 5.5 × 10^7 RIU, respectively. The experimental study on the drug susceptibility of living cells is carried out by the system. The realtime changes of cell refractive index during the biological evolution of cisplatin and paclitaxel in Ramos cells and Jeko- 1 cells are studied, and the consistency of refractive index changes with its pharmacological mechanism is verified.

关 键 词：生物光学 折射率传感 石墨烯 活细胞 药物敏感性 

分 类 号：O436[机械工程—光学工程]