HPLC法测定黄七化瘀丸中三七皂苷R1、人参皂苷Rg1及人参皂苷Rbl的含量  

Determination of Notoginsenoside R1, Ginsenoside Rg1, and Ginsenoside Rb1 in Huangqi Huayu Wan by HPLC

作　　者：田蜜[1] 陈芳[1] 贺建昌[1] TIAN Mi;CHEN Fang;HE Jianchang(Medicine Department of Kunming General Hospital of Chengdu Military Command,Kunming 650032,China)

机构地区：[1]成都军区昆明总医院药学部,昆明650032

出　　处：《吉林中医药》2018年第11期1319-1322,共4页Jilin Journal of Chinese Medicine

基　　金：军队医疗机构制剂标准提高计划专项课题重点项目-“成都战区云南域非标准制剂质量提升关键技术的系统研究”(14ZJZ18)

摘　　要：目的建立高效液相色谱法测定黄七化瘀丸中三七皂苷R1、人参皂苷Rg1及人参皂苷Rbl含量。方法色谱柱Symmetry C18(4.6×250 mm,5μm),流动相:乙腈-0.02 mol/L的磷酸二氢钾溶液(20:80),流速:1.0 mL/min,检测波长:203 nm,进样量10μL。结果三七皂苷R1、人参皂苷Rg1及人参皂苷Rbl在2.1675～86.70μg/mL范围内时,与峰面积呈良好的线性关系(R2=0.9996),平均回收率为87.88%。结论 HPLC法测定三七皂苷R1、人参皂苷Rg1及人参皂苷Rbl含量的方法简便,灵敏度高,重复性好,可用于胃肠安中三七皂苷R1、人参皂苷Rg1及人参皂苷Rbl的含量测定。Objective To establish an HPLC method for determination of Notoginsenoside R1, Ginsenoside Rg1 and Rb1 content in Huangqi Huayu Wan. Methods Symmetry C18（4.6×150 mm, 5μm）was used. The mobile phase was acetonitrile-0.02 mol/L monopotassium phosphate solution（20：80）. The flow rate was 1.0 mL/min and the detection wavelength was 230 nm.The injection volume was 10 μL. Results Within the range from 2.1675 to 86.70 μg/mL, berberine hydrochloride presented a ideal linear relationship with the peak areas（R2=0.9996）. The average recovery was 87.88%. Conclusion This HPLC method for determining the content of Notoginsenoside R1, Ginsenoside Rg1 and Rb1 is simple, highly sensitive, and reproducible, which can be used for the quality of Notoginsenoside R1, Ginsenoside Rg1 and Rb1 content in Huangqi Huayu Wan.

关 键 词：黄七化瘀丸 三七皂苷R1 人参皂苷RG1 人参皂苷RB1 高效液相色谱法 含量 

分 类 号：R927.2[医药卫生—药学]