长链非编码RNA MAPKAPK5-AS1在肺癌组织中的表达及临床意义  

Expressions and clinical implications of long non-coding RNA(lncRNA) MAPKAPK5-AS1 in lung cancer tissues

作　　者：孙一奎[1] 赵枫[1] 吕绮 王卫华[1] SUN Yi-kui;ZHAO Feng;LV Qi;WANG Wei-hua(The Affiliated Hospital of Medical School of Ningbo University,Ningbo,Zhejiang 315000,China)

机构地区：[1]宁波大学医学院附属医院

出　　处：《中国卫生检验杂志》2018年第22期2731-2733,共3页Chinese Journal of Health Laboratory Technology

基　　金：浙江省宁波市自然科学基金项目(2015A610237)

摘　　要：目的 研究长链非编码RNA （long ncRNA,lncRNA） MAPKAPK5-AS1在肺癌组织中的表达及临床意义。方法 收集143例经病理明确诊断的肺癌患者癌组织及配对癌旁正常肺组织标本和临床资料,real time PCR法检测组织中lncRNA MAPKAPK5-AS1的表达,分析其临床意义,通过Kaplan-Meier生存曲线法和Log Rank检验法分析其对患者预后意义。结果 143例肺癌组织中MAPKAPK5-AS1相对表达水平（0. 012 2±0. 000 7）明显比癌旁正常肺组织中相对表达水平（0. 007 1±0. 000 4）高,差异有统计学意义（t=5. 727,P 〈0. 01）;高表达MAPKAPK5-AS1与肺癌患者年龄、TNM分期、淋巴结转移、远处转移均密切相关（P值均〈0. 05）,并且与预后不良密切相关（t=3. 97,P 〈0. 05）。结论 肺癌组织中高表达MAPKAPK5-AS1与肿瘤转移密切相关,是潜在肺癌预后生物学指标之一。Objective To study the expression and clinical implications of lncRNA MAPKAPK5 - AS1 in lung cancer tissues. Methods Lung cancer tissues and paired normal lung tissues nearby lung cancer tissues were collected, along with patient＇s clinical data. MAPKAPK5 - AS1 expression in these tissues was detected by real time PCR. Prognostic significance was analyzed by Kaplan - Meier survival curve and Log Rank test. Results MAPKAPK5 - AS1 expression was higher in the lung cancer tissues （0.012 2±0. 000 7） than the paired normal lung tissues（0. 007 1±0.000 4）, with the difference of statistical significance （t = 5. 727, P 〈 0.01 ）. High expressed MAPKAPK5 - AS1 was closely related to patient＇s age, TNM stage, lymphatic metastasis, and distant metastasis （ P 〈 0.05 ）, along with poor prognosis （ t = 3.97, P 〈 0.05 ）. Conclusion High expression of MAPKAPK5 - AS1 in the lung cancer tissues is intimately related to cancer metastasis, and may be a potential biomarker for lung cancer.

关 键 词：肺癌 长链非编码RNA MAPKAPK5-AS1 

分 类 号：R446.6[医药卫生—诊断学]