传染性单核细胞增多症外周血淋巴细胞亚群和免疫球蛋白变化特点分析  

Changes of lymphocyte subsets and immunoglobulin in peripheral blood of children with infectious mononucleosis

作　　者：符佳[1] 张玉凤[1] 朱欣欣 谢燕[1] 邓慧玲[1] 张瑜[1] FU Jia;ZHANG Yu-feng;ZHU Xin-xin;XIE Yan;DENG Hui ling;ZHANG Yu(Department of Infection,Xi＇an Children＇s Hospital,Shaanxi Xi＇an 710003,China)

机构地区：[1]西安市儿童医院感染二科,陕西西安710003

出　　处：《中国妇幼健康研究》2018年第11期1472-1475,共4页Chinese Journal of Woman and Child Health Research

摘　　要：目的分析儿童EB病毒(EBV)感染致传染性单核细胞增多症的外周血淋巴细胞亚群和免疫球蛋白的变化特点。方法随机选取2017年1至12月西安市儿童医院感染二科诊治的40例感染EBV致传染性单核细胞增多症患儿作为研究组,选取同期体检健康儿童30例作为对照组,通过免疫散射比浊法测量两组儿童的IgA、IgG、IgM表达水平,通过流式细胞术测量两组儿童的B淋巴细胞和CD3^+、CD4^+、CD8^+淋巴细胞及自然杀伤细胞的数量,并对结果进行分析。结果研究组的免疫球蛋白IgA、IgG均显著高于对照组(t值分别为9.29、5.56,均P<0.05);研究组的淋巴细胞和CD3^+、CD8^+淋巴细胞及白细胞数量均显著高于对照组(t值分别为18.62、19.08、17.46、23.27,均P<0.05),研究组B淋巴细胞数量显著低于对照组(t=22.75,P<0.05)。结论 CD8^+淋巴细胞数量急剧增加可能是感染EBV致传染性单核细胞增多症患儿淋巴细胞急剧增加的重要因素,且患儿免疫球蛋白IgA、IgG急剧增加,因此通过检测淋巴细胞亚群对诊断感染EBV致传染性单核细胞增多症具有一定意义。Objective To analyze the changes of lymphocyte subsets and immunoglobulin in peripheral blood of children with infectious mononucleosis caused by EB virus（EBV）infection.Methods From January to December 2017,40 children with infectious mononucleosis caused by EBV in Xi＇an Children＇s Hospital were randomly selected as study group,and 30 healthy children in the same period were selected as control group.The expressions of IgA,IgG and IgM in two groups of children were measured by immunization turbidimetry,and the number of B lymphocyte,CD3~＋,CD4~＋,CD8~＋ lymphocytes and NK cells of two groups of children were measured by flow cytometry.Results The immunoglobulin IgA and IgG in the study group were significantly higher than those in the control group（t value was 9.29 and 5.56,respectively,both P〈0.05）,and the numbers of lymphocyte,CD3~＋and CD8~＋ lymphocytes and leukocyte in the study group were significantly higher than those in the control group（t value was 18.62,19.08,17.46 and 23.27,respectively,all P〈0.05）,but the number of B lymphocyte in the study group was significantly lower（t=22.75,P〈0.05）.Conclusion The rapid increase in the number of CD8~＋ lymphocyte may be an important factor in the rapid increase of lymphocytes in children with infectious mononucleosis caused by EBV,and immunoglobulin IgA and IgG in patient children increase rapidly.Therefore,the detection of lymphocyte subsets is of certain significance in the diagnosis of infectious mononucleosis caused by EBV infection.

关 键 词：EB病毒 传染性单核细胞增多症 淋巴细胞亚群 免疫球蛋白 儿童 

分 类 号：R725.5[医药卫生—儿科]