哈萨克族和汉族子痫前期患者的血脂变化与胎盘组织中脂蛋白酯酶表达的相关研究  

The research on the changes of blood lipids and the expression of lipoprotein lipase in placenta between Kazakh and Han nationality pregnant women with preeclampsia

作　　者：李晶[1,2] 李祥雯[1] 朱启英[1] LI Jing;LI Xiang-wen;ZHU Qi-ying(Department of Obstetrics,First Affiliated Hospital of Xinjiang Medical University,Xinjiang,Urumqi,830054;Xinjiang Medical University,Xinjiang,Urumqi,830011)

机构地区：[1]新疆医科大学第一附属医院产科,乌鲁木齐830054 [2]新疆医科大学,乌鲁木齐830011

出　　处：《中国优生与遗传杂志》2018年第11期63-66,共4页Chinese Journal of Birth Health & Heredity

基　　金：新疆维吾尔自治区自然科学基金(2016D01C278)

摘　　要：目的探究哈萨克族和汉族子痫前期产妇的血脂变化与脂蛋白酯酶(LPL)在胎盘中表达的相关性。方法选取2016年9月至2017年3月在新疆医科大学第一附属医院产科确诊入住的100例子痫前期产妇为研究对象,依据民族不同分为各50例产妇的哈萨克族子痫前期组和汉族子痫前期组,另随机抽取同期汉族正常产妇50例作为对照组。采用酶法检测血脂浓度,ELISA法检测血清LPL浓度,RT-PCR和WesternBlot法检测胎盘组织LPL基因及蛋白表达水平。结果哈萨克族及汉族子痫前期组产妇血脂中总胆固醇(TC)、甘油三酯(TG)及低密度脂蛋白(LDL)浓度均高于对照组(P<0.05),高密度脂蛋白(HDL)浓度低于对照组(P<0.05),两子痫前期组产妇血脂中LDL、HDL及TG浓度比较存在明显差异(P<0.05);两子痫前期组产妇血清LPL浓度均低于对照组(P<0.05),哈萨克族子痫前期组产妇的血清LPL浓度低于汉族子痫前期组(P<0.05);哈萨克族和汉族子痫前期组产妇胎盘组织LPL基因及蛋白的表达水平均低于对照组(P<0.05);两子痫前期组产妇的血清LPL浓度与HDL(r=0.766、0.661)、胎盘组织LPL蛋白(r=0.613、0.427)呈显著正相关(P<0.05),与TG呈显著负相关(r=-0.732、-0.594,P<0.05)。结论哈萨克族和汉族子痫前期产妇血脂变化受多种因素调控,且与产后胎盘组织中LPL的表达具有相关性。Objective： To investigate the relevance between the expression of lipoprotein lipase （LPL） in serum and in placenta of Kazakh and Han nationality preeclampsia patients. Methods： 50cases of Kazakh preeclampsia pregnancies, 50cases of Han preeclampsia pregnancies and 50cases normal pregnancies were enrolled from September 2016 to March 2017 in the first affiliated hospital of Xinjiang medical university. Venous blood was collected to detect the levels of lipids and LPL in serum by enzymic method and ELISA. The levels of LPL mRNA and protein in placenta were detected by RT-PCR and Western blot. Results： The levels of serum lipids were increased in both Kazakh and Han preeclampsia pregnancies while the concentration of high-density lipoprotein （HDL） was decreased （P〈0.05） when compared with control group. At the same time, the low- density lipoprotein （LDL） , high-density lipoprotein （HDL） , and triglyceride （TG） levels in Kazak patients are also significantly different from those in Han women （P〈0.05） . The levels of serum LPL and LPL mRNA and protein in placenta were significantly decreased in preeclampsia pregnancies （P〈0.05） . However, there was no significant difference in the expression of LPL in the placental of 2 ethnic patients. The concentration of serum LPL were positively related to that of HDL （ r=0.766; 0.661, P〈0.05） , and positively related to that of LPL protein in placenta （r=0.613; 0.427, P〈0.05） , and negatively related to that of serum triglyceride （TG） （r=-0.732; -0.594 P〈 0.05） in preeclampsia pregnancies. Conclusions： The blood lipid changes in Kazakh and Han patients may be regulated by various factors, and are related to the expression of LPL in placenta tissue.

关 键 词：哈萨克族 子痫前期 血脂 脂蛋白酯酶 

分 类 号：R714.245[医药卫生—妇产科学]