上转换纳米粒子结合DNA链式扩增技术在阿霉素光控释放中的应用  

Upconversion Nanoparticles Decorated with Hybridization Chain Reaction DNA for Near-infrared Light Controlled Release Doxorubicin

作　　者：于畅 邵帅 李贺杰 刘国锋 丁彬彬 马平安 林君[1] YU Chang;SHAO Shuai;LI Hejie;LIU Guofeng;DING Binbin;MA Ping′an;LIN Jun(a State Key Laboratory of Rare Earth Resource Utilization,Changchun Institute ofApplied Chemistry,Chinese Academy of Sciences,Changchun 130022,China;b University of Chinese Academy of Sciences,Beijing 100049,Chin;c School of Chemistry and Environmental Engineering,Changchun Universityof Science and Technology,Changchun 130022,China;d Department of Otorhinolaryngology Head and Neck Surgery,Second Hospital of Jilin University,Changchun 130041,China;e College of Physics,Jilin University,Changchun 130012,Chin;f University of Science and Technology of China,Hefei 230026,China)

机构地区：[1]中国科学院长春应用化学研究所,稀土资源利用国家重点实验室,长春130022 [2]中国科学院大学,北京100049 [3]长春理工大学化学与环境工程学院,长春130022 [4]吉林大学第二医院,耳鼻喉-头颈外科,长春130041 [5]吉林大学物理学院,长春130012 [6]中国科学技术大学,合肥230026

出　　处：《应用化学》2018年第12期1485-1491,共7页Chinese Journal of Applied Chemistry

基　　金：国家自然科学基金(51672268;51472233;51332008;51720105015;51628201;21521092);中国科学院前沿科学重点研究项目(YZDYSSW-JSC018);吉林省科技引导计划(20170101188JC;20170414003GH)项目资助~~

摘　　要：纳米技术的发展使得纳米材料可以通过不同的表面包覆和修饰而在生物医药中发挥应用。构建简单、经济、药物释放可控的生物相容性纳米药物仍是纳米生物化学领域的重点。我们构建的纳米载药体系(DDS)以Na YF4∶Yb/Tm上转换纳米粒子为载体,在其表面通过光致断键型小分子4,5-二甲氧基-2-硝基苯基乙酮(DMNPE)连接一段短单链DNA,利用DNA链式扩增技术(HCR)来调节纳米粒子最终修饰的双链DNA的总量,从而控制对抗癌药物阿霉素(Dox)的担载量,在980 nm激光照射下上转换纳米粒子发射可切断DMNPE连接的近紫外光,协同胞内DNA酶的作用达到对药物的可控释放。由于近红外光照对生物组织具有较好的穿透能力,此体系能够对病灶位置有更好的光靶向性从而减少药物的毒副作用。Nanotechnology has allowed various coatings and modifications to nanostructures for applications of biomedicine. The constructions of simple, economical, controllable and biocompatible drug delivery systems（DDS） are still in demand. Here, we report a NaYF 4 ∶ Yb/Tm nanoparticle-based DDS that anticancer drug doxorubicin（Dox） could be quantitatively carried by double stranded DNA thereon that self-assembled through a hybridization chain reaction（HCR）. The HCR DNA was absorbed on the surface of upconversion nanoparticles（UCNPs） through linkage to photocleavable 1-（4,5-dimethoxy-2-nitrophenyl） diazoethane（DMNPE） molecules , which can be cleaved by near ultraviolet light emitted by UCNPs under 980 nm laser light excitation. The controllable release of model drug Dox by near-infrared（NIR） should facilitate selective cytotoxicity to target region and reduced side effects and could prove promising in biomedical applications.

关 键 词：上转换纳米粒子 DNA链式扩增 控制释放 盐酸阿霉素 

分 类 号：O611.4[理学—无机化学]