三(4-甲基苯甲酸)纤维素酯作为色谱固定相的性能研究  

Cellulose Tris(4-Methylbenzoate) as Chromatographic Stationary Phase and its Characterization

作　　者：施介华[1] 杨根生[1] 郑云峰[1] 

机构地区：[1]浙江工业大学药学院,浙江杭州310032

出　　处：《高校化学工程学报》2002年第4期373-377,共5页Journal of Chemical Engineering of Chinese Universities

基　　金：本项目系浙江省分析测试基金资助项目(00035)

摘　　要：采用催化合成法合成三（4－甲基苯甲酸）纤维素酯，并负载于Gas Chrom Q载体上，用不同类型的探针分子表征其作为气相色谱固定相时的吸附性能。实验结果表明，不同类型探针分子在三（4－甲基苯甲酸）纤维素酯上具有不同的吸附热（－△Ha)，这表明探针分子与固定相之间发生不同的相互作用力，即π-π相互作用，氢键作用以及偶极－偶极相互作用等。并且,在三（4－甲基苯甲酸）纤维素酯／GDX103的色 谱固定相上成功地分离了脂肪酸（C1－C6），特别是较好地分离丙醇和丁醇的六个异构体。The cellulose tris(4-chlorobenzoate) was synthesised by the catalytic method. The absorption capability of Gas Chrom Q coated with cellulose tris(4-chlorobenzoate) used as a chromatographic stationary phase was characterized by a variety of probe molecules. The experimental results showed that values of the heat of adsorption (-DHa) of a variety of probe molecules on Gas Chrom Q coated with cellulose tris(4-chlorobenzoate) were different. It indicates that the various kinds of interactions between probe molecules and chromatographic stationary phase occur which including p-p interaction, hydrogen-bonding interaction, dipole-dipole interaction and so on. The organic functional groups on the cellulose tris(4-methylbenzoate) such as phenyl and ester carbonyl groups have important roles in the chromatographic separation process. C1~C6 aliphatic alcohols were successfully separated on glass column packed with GDX10 coated with cellullose tris(4-chlorobenzoate). The separation efficiency of six isomers of propanol and butanol was also good.

关 键 词：三(4-甲基苯甲酸)纤维素酯 色谱固定相 吸附性能 脂肪醇 分离 

分 类 号：O658[理学—分析化学] O623.41[理学—化学]