抗生素单一或联合用药对绿脓杆菌PA103释放内毒素的影响  

Effects of different type and combinations of antibiotic on endotoxin release from pseudomonas aeruginosa 103

作　　者：王顺宾[1] 吴伯瑜[1] 陈晓东[1] 杨际慧[1] 黄逢元[1] 

机构地区：[1]福建医科大学附属协和医院烧伤研究所,350001

出　　处：《中国民政医学杂志》2002年第5期257-259,共3页Medical Journal of Chinese Civil Administration

基　　金：福建省科委资助项目 (编号 98-Z - 16 2 )

摘　　要：目的 :观察不同种类抗生素低浓度下单一或联合用药 ,对细菌释放内毒素的影响。方法 :选择临床上常用的几种抗生素 (环丙沙星、丁胺卡那霉素、伊米配能、头孢氨噻肟 ) ,以 0 5倍最小抑菌浓度 (0 5×MIC)单一或联合于体外作用于绿脓杆菌PA1 0 3 ,观察抗生素的杀菌作用、检测培养上清液内毒素水平和细菌形态学改变。结果 :不同种类抗生素作用于绿脓杆菌PA1 0 3后 ,在抑制、杀灭细菌 ,引起细菌形态学改变的同时 ,能不同程度诱导细菌释放一定量的内毒素 ;其中头孢氨噻肟 ,环丙沙星诱导释放的内毒素较多 ,伊米配能诱导释放的内毒素较少。联合用药后杀菌效果优于单一用药组 ,同时诱导释放的内毒素也较少。结论 :诱导绿脓杆菌PA1 0 3释放内毒素与抗生素的种类有关 。Objective:To evaluate the release of endotoxin from pseudomonas aeruginosa 103 after incubated with different antibiotics alone or combined Method:The endotoxin level of PA103 exposed to the antibiotics frequently used in clinical with 0 5×MIC was studied in vitro in relation to the antibiotic-effect on viable counts and morphological feature of the cultures Results:The study shows that different antibiotics induce diferent endotoxin release from PA103 during the morphological features were changed or killed Cefotaxime and Ciprofloxacin induced the higher release of endotoxin,whereas Imipenem induced the lower release of endotoxin compared to the controls Asignificant reduce in endotoxin prodution was also noticed in the combinations of two antibiotics compared to the alone group Conclusions:The endotoxin release from PA103 was related to the types of antibiotics The endotoxin levels in the combined group were lower than in the alone group [

关 键 词：绿脓杆菌 内毒素 抗生素 细菌 联合用药 单一用药 

分 类 号：R978.1[医药卫生—药品] R378.991[医药卫生—药学]