PINⅡ基因通过花粉管通道法转化水稻的研究  

Studies on culture of insect - resistant rice by transforming rice with PIN Ⅱ gene via the pollen - tube pathway

作　　者：罗明[1] 罗忠训[1] 朱彩章[2] 周勇[1] 

机构地区：[1]湖北大学生命科学学院,湖北武汉430062 [2]湖北省种子集团公司,湖北武汉430070

出　　处：《湖北大学学报（自然科学版）》2002年第3期259-262,共4页Journal of Hubei University：Natural Science

摘　　要：利用花粉管通道法,用含抗除草剂基因bar及抗虫基因pin2的质粒pTW-α转化中籼HD357。经用除草剂Basta进行筛选,得到5株抗性苗。对其进行初步PCR检测,结果表明,有4株PCR结果呈阳性。用PINⅡ基因探针对其进行Southern杂交分析,有2株表现出有杂交信号,说明外源基因已整合转入到水稻基因组中,抗虫性实验尚待继续进行。Via pollen - tube pathway the indica rice HD - 357 was transformed with plasmid pTW - α containing Bar gene and PIN Ⅱgene. Among five herbicide - resistant plants, four of them can generate 408 bp bands with Bar primers by PCR test, two of them got hybridization bands in the Southern blot hybridization analysis by using 1.5 kb PIN Ⅱ probe. These results indicates that the foreign gene had been transformed into plants genome. The insect resistance bioassay will be continued in the future.

关 键 词：PINⅡ基因 花粉管通道法 转基因水稻 抗除草剂基因 抗虫基因 良种培育 

分 类 号：S511[农业科学—作物学] Q943.2[生物学—植物学]