丝素/纳米纤维素晶须/壳聚糖多孔复合支架的应用  

Composite Silk Fibrin/cellulose Nano Whiskers-chitosan Porous Scaffolds for Bone Tissue Engineering Applications

作　　者：谭卫琳 张琦[1,2] 何健新[1,2] 韩啟明 崔世忠[1,2,3] 邵伟力[3] 

机构地区：[1]中原工学院河南省功能性纺织材料重点实验室,河南郑州450007 [2]中原工学院纺织服装产业河南省协同创新中心,河南郑州450007 [3]天津工业大学纺织学院,天津300387

出　　处：《材料科学与工程学报》2015年第6期868-872,共5页Journal of Materials Science and Engineering

基　　金：国家自然科学基金资助项目(51203196);河南省教育厅科学技术研究重点资助项目(14A540003;14A540006);郑州市普通科技攻关资助项目(141PPTGG400)

摘　　要：以冷冻干燥法制备多孔丝素(SF)支架,利用层层自组装将纳米纤维素晶须(CNW)和壳聚糖(CS)交替组装到多孔SF支架上得到SF/CNW-CS多孔复合支架。对SF/CNW-CS多孔复合支架的形貌和机械性能进行了表征。以MG-63细胞进行体外培养评估SF及SF/CNW-CS多孔复合支架的细胞相容性,MTT比色法和荧光图像的测试结果表明,与SF多孔支架相比,MG-63细胞在SF/CNW-CS多孔复合支架上的增殖、粘附和分化功能最高。因此,SF/CNW-CS支架有望成为骨组织工程的理想材料。Silk fibrin/cellulose whiskers-chitosan(SF/CNW-CS)composite scaffolds with superior mechanical properties and biological compatibility were prepared by layer-by-layer self-assembly of CNW and CS into porous SF scaffolds that were fabricated by freeze-drying.The morphology and mechanical properties of SF/CNW-CS composite scaffolds were characterized using different technical methods.The influence of SF and SF/CNW-CS scaffolds on cellular biocompatibility was assessed in vitro culture.The results indicated that SF/CNW-CS composite scaffolds well supported the adhesion and proliferation of MG-63 cells,and exhibited an excellent function compare with porous SF scaffold via MTT and fluorescence images.The SF/CNW-CS scaffolds might be a promising material for bone tissue engineering.

关 键 词：多孔丝素支架(SF) 纳米纤维素晶须(CNW) 层层自组装 骨组织工程 

分 类 号：TB33[一般工业技术—材料科学与工程] R318.08[医药卫生—生物医学工程]