八月红梨叶片不定芽诱导研究  

Regeneration of adventitious bud from leaves of 'Bayuehong'pear

作　　者：徐凌飞[1] 马锋旺[1] 王喆之[2] 任小林[1] 

机构地区：[1]西北农林科技大学园艺学院,陕西杨陵712100 [2]陕西师范大学生命科学学院,陕西西安710062

出　　处：《西北农林科技大学学报（自然科学版）》2002年第4期73-75,共3页Journal of Northwest A&F University(Natural Science Edition)

基　　金：西北农林科技大学青年科研专项基金资助项目

摘　　要：采用正交试验设计研究了基本培养基、BA和 IAA 3个因素及其组合对八月红梨 (Pyrus communisL .)叶片不定芽再生的影响。结果表明 ,基本培养基的种类对叶片不定芽的诱导起主要作用 ,诱导叶片再生的最优组合是 NN6 9+BA 5 .0 m g/L +IAA 0 .5 mg/L ;用同质量浓度葡萄糖代替蔗糖 ,对叶片再生频率的影响不显著 ,用白砂糖代替蔗糖 ,显著降低了叶片再生频率 ;Ag NO3质量浓度在 0 .1～ 1.5 mg/L时 ,对叶片再生有促进作用 ,培养基中附加 Ag NO30 .5 mg/L 。The basal medium,BA,and IAA were used to compare their effect on adventitious bud induction from leaves of 'Bayuehong'pear by using the orthogonal experiment design.The results showed that the basal medium played a determinative role in initating the adventitious buds.NN69+BA 5.0 mg/L+IAA 0.5 mg/L was the best combination for leave regeneration.No effect on regeneratioon efficiency of leave was found by substituting equal concentration glucose for sucrose in NN69 medium,while replacing sucrose with granulated sugar greatly reduced regeneration efficiency.AgNO 3 enhanced the regeneration efficiency of leave while its concentration varied from 0.1 to 1.5 mg/L,and the most effective concentration of AgNO 3 was 0.5 mg/L.

关 键 词：八月红 梨 叶片 不定芽 诱导 

分 类 号：S661.2[农业科学—果树学]