蔗糖合酶基因启动子克隆及其转基因水稻植物中特异性表达  

Cloning and Specific Expression the Rice Sucrose Synthase Gene Promoter in Transgenic Rice Plants

作　　者：李永春[1] 张宪银[1] 薛庆中[1] 

机构地区：[1]浙江大学农学系

出　　处：《作物学报》2002年第5期586-590,共5页Acta Agronomica Sinica

基　　金：国家 8 63计划 (10 1-0 1-0 1-3 );国家自然科学基金 (3 9870 42 );浙江省 8812计划资助

摘　　要：以水稻品种“明恢 6 3”基因组 DNA为模板 ,用 PCR方法克隆出水稻蔗糖合酶基因起始密码子以前 (包括第一内含子 )的上游序列 RSP1及其不包括第一内含子的上游调控序列 RSP2。测序结果显示 ,在重要的功能区段上 ,克隆的序列与 Wang(1992 )等的报道基本一致。构建了由 RSP1和 RSP2驱动报告基因 Gus的植物表达载体 ,并用于农杆菌介导法水稻遗传转化。对水稻品种“秀水 11”转基因植株各部位的 GUS组织化学分析表明 :RSP1和 RSP2都可以驱动Gus基因在根、茎、叶及颖壳中高效特异表达 ,但在胚和胚乳中不表达。作者认为 。According to the pubilished 5′ upstream sequence of the rice sucrose synthase gene, two pairs of specific primers, which involved RSP1 with an intron and RSP2 without intron, were designed and amplified by using PCR technique from genomic DNA template of an Indica rice variety 'Minghui 63'. There were no differences between the cloned sequences and the sequences published by Wang et al. 1992 in dominant functional regions. Both RSP1 and RSP2 link up with Gus gene respectively in the Agrobacterium binary vectors for the Agrobacterium mediated transform of a rice variety 'Xiushui 11'. Histochemical analysis of Gus activity in various tissues showed that the Gus gene driven by RSP1 or RSP2 gave strong expression in the roots, stems, leaves and seed coats, but did not in the embryo and endosperm tissues, based on which we suggested that the specific expression of the sucrose synthase gene is favorable to improving the comestible safe when using the promoter to rice molccular breeding.

关 键 词：蔗糖合酶基因 启动子 克隆 转基因 水稻 植物 特异性表达 

分 类 号：Q943.2[生物学—植物学]