两种剂量氟碳部分液体通气对吸入伤犬铜蓝蛋白及肺内血浆蛋白外漏的影响  

Effect of partial liquid ventilation with two different doses of perfluorocarbon on serum protein and ceruloplasmin in dogs with inhalation injury

作　　者：朱峰[1] 郭光华[1] 胡庆宏[1] 熊龙[1] 李国辉[1] 

机构地区：[1]江西医学院第一附属医院烧伤研究所,江西南昌330006

出　　处：《中国危重病急救医学》2002年第9期529-531,共3页Chinese Critical Care Medicine

基　　金：国家自然科学基金资助项目 (No.3 9760 0 73 ) ;江西省主要学科跨世纪学术和技术带头人培养计划项目 (No.K990 5 0 2 )

摘　　要：目的 :对比观察两种剂量氟碳 (PFC)部分液体通气 (PL V)对吸入性损伤 (INI)犬血丙二醛 (MDA)、铜蓝蛋白 (CP)及肺泡灌洗液 (BAL F)和肺匀浆中铜蓝蛋白、血浆蛋白的影响。方法 :制作 15条犬蒸气 INI模型并分成 PFC 6 m l/ kg组 (PFC 6组 )和 12 ml/ kg组 (PFC 12组 )。将 PFC(C1 0 F1 8,英国产 )缓慢注入肺内 ,实施PL V,于治疗 2小时后抽静脉血测 MDA、CP;并测 BAL F、肺匀浆中 CP、总蛋白 (TP)、白蛋白 (AL B)、球蛋白(GL B)及白球比 (A/ G)。结果 :PL V后 2小时 ,PFC 6组与 PFC 12组血 MDA、CP比较均无显著性差异(P均 >0 .0 5 ) ,但均较各自的致伤前明显下降 (P均 <0 .0 5 )。 BAL F中 ,PFC 12组的 TP、AL B、GL B及 A/ G均较 PFC 6组明显降低 (P均 <0 .0 5 ) ,而 CP无明显改变。肺匀浆中 ,PFC 12组的 TP、AL B、GL B及 A/ G均较 PFC 6组明显降低 (P均 <0 .0 5 ) ,而 CP则明显增高 (P<0 .0 5 )。结论 :PL V可能通过抗脂质过氧化等作用而减少血浆蛋白外漏 ,降低肺水肿的发生 ,并呈现剂量效应趋势。Objective:To investigate effects of partial liquid ventilation(PLV) in two different doses of perfluorocarbon(PFC) on ceruloplasmin(CP) and serum protein in dogs with inhalation injury.Methods:Dogs ( n =15) with inhalation injury were treated with PLV(instillation of PFC slowly into the lung).The concentrations of CP,malondialdehyde(MDA) in serum and total protein(TP),albumin(ALB),globulin (GLB),CP in bronchoalveolar lavage fluid and lung homogenate were detected 2 hours after PLV.Results:Two hours after PLV,when compared to PFC6 group,CP,MDA in serum had no changes in PFC12 group(all P >0 05);TP,ALB,GLB in bronchoalveolar lavage fluid decreased significantly in lung PFC12 group compared with PFC6 group(all P <0 05),but CP had no significant change;TP,ALB,GLB in homogenate decreased remarkably in PFC12 group compared with PFC6 group(all P <0 05),meanwhile CP increased significantly ( P <0 05).Conclusions:The treatment with PLV may decrease leakage of serum protein in dogs with inhalation injury by antilipid peroxidant,thus decrease lung edema.The effect is dosedependent.

关 键 词：氟碳 吸入性损伤 部分液体通气 血浆蛋白 铜蓝蛋白 丙二醛 剂量-效应 脂质过氧化 

分 类 号：R644[医药卫生—外科学]