2株鸡传染性法氏囊病病毒超强毒株的分离鉴定  

Separation and Identification of Two Very Virulent Strains of Infectious Bursal Disease Virus

作　　者：孙继国[1] 张艳英[1] 郑世学[1] 张曼夫[2] 

机构地区：[1]河北农业大学动物科技学院,河北保定071001 [2]中国农业大学生物学院,北京100094

出　　处：《中国兽医学报》2002年第5期442-444,共3页Chinese Journal of Veterinary Science

基　　金：河北省自然科学基金资助项目 ( 30 0 115 )

摘　　要：用分离的疑为鸡传染性法氏囊病病毒 (IBDV)超强毒株 (vv IBDV) He B- lf和 He B- bd接种 SPF鸡 ,36 h后接种鸡开始发病 ,发病率为 10 0 %,死亡率为 5 0 %以上。剖检可见与野外病例相同的病理变化 ,如肌肉、心脏、腺胃出血 ,法氏囊水肿或呈“紫葡萄”样外观。经血清学试验、毒力试验、鸡胚接种试验、分子生物学和电镜形态学观察均证明该两分离物为超强毒株 ,从而证明河北省鸡群中存在不同于经典 IBDV的超强毒株。HeB-lf and HeB-bd, the two suspected virulent strains of IBDV, were inoculated to the SPF chicken, 36 hours postinoculation they were found to be infected with the infectious rate of 100% and the death rate of more than 50%. The inoculated chickens occured the similar pathologic changes to the field case. By the serological examination,virulence examination, and electron microscope observation, the two strains were proved to be very virulent strains different from classic IBDV.

关 键 词：鸡 传染性 法氏囊病 病毒 超强毒株 分离鉴定 序列分析 

分 类 号：S858.31[农业科学—临床兽医学]