肾型鸡传染性支气管炎病毒X株S1基因序列的测定及同源性分析  

Sequencing and Homology Analysis of the S1 Gene of Nephropathogenic Strain X

作　　者：赵宝华[1] 李尚波[2] 王文成[2] 尹广东[2] 卫广森[2] 许崇波[3] 

机构地区：[1]河北师范大学生命科学学院,河北石家庄050016 [2]辽宁省益康生物制品厂,辽宁辽阳111000 [3]宁夏大学生物工程系,宁夏银川750021

出　　处：《中国兽医学报》2002年第5期445-447,共3页Chinese Journal of Veterinary Science

基　　金：河北师范大学博士后基金资助项目 ( 2 0 0 1B0 3)

摘　　要：根据国外已发表的鸡传染性支气管炎病毒 (IBV) S1基因序列 ,设计了 2对引物并以 RT- PCR特异性扩增出IBV X株的 S1基因 ,基因产物大小为 1.6 4kb,与设计相符 ,对其进行序列测定后 ,与标准毒株 H5 2、H12 0、M41、BEAU和澳大利亚 T株的 S1基因进行同源性比较。结果表明 ,X株与 H5 2、H12 0、M41、BEAU和澳大利亚 T株的核苷酸序列的同源性分别为 75 .8%、76 .1%、76 .3%、75 .5 %和 76 .9%,由此可以看出 ,IBV X株与标准毒株在 S1基因上存在较大差异。Two pair of primers for amplifying the S1 gene of avian infectious bronchitis virus (IBV)had been designed,with which the predicted 1.62 kb fragment had been amplified by RT-PCR and RT-NPCR from Nephropathogenic Strain X.The S1 gene of Nephropathogenic Strain X was sequenced and compared with standard strains such as H52,M41,H120,BEAU and T.The results showed that the homologies of nucleic acids between X strain and H52,M41,H120,BEAU,T strains were 76.1%,76.3%,75.8%, 75.5% and 76.9% respectively.The difference of the S1 gene of Nephropathogenic Strain X with those of other IBV standard strains had been proved.

关 键 词：肾 鸡 传染性 支气管炎 病毒 X株 S1基因 同源性分析 核苷酸序列 

分 类 号：S858.31[农业科学—临床兽医学] Q78[农业科学—兽医学]