蛋白内含子与蛋白剪接  

Intein and Protein-splicing

作　　者：魏令波[1] 费云标[1] Paul X Liu 

机构地区：[1]中国科学院遗传与发育生物学研究所,北京100080 [2]Department of Biochemistry & Molecular Biology Dalhousie University

出　　处：《中国生物工程杂志》2002年第4期12-16,共5页China Biotechnology

摘　　要：蛋白内含子和蛋白剪接是蛋白质研究的前沿领域。重点介绍了蛋白内含子的结构和蛋白剪接机理的最新研究成果 ;蛋白内含子如同RNA剪接中的内含子 ,也是一类可移动的遗传元件 ;Intein is a self splicing protein and can excise itself from the host protein and at same time splices/joins then the two flanking host protein sequences,named the extein by a normal peptide bond.This process is termed the protein splicing.Therefore,protein splicing is a form of posttranslational processing and could be viewed as the equivalent of intron and RNA splicing.A intein can be divided three independent parts,N terminal and C terminal domain located at two ends and endonuclease domain located at the middle of the intein.Protein splicing involves a series of four intramolecular reaction,the first three of which occur at a singlecatalytic centre of the N terminal and C terminal domain of intein.Endonuclease domain has homing function and make the intein become a mobile genetic element.Intein has been practical uses in protein engineering.\;

关 键 词：蛋白内含子 蛋白剪接 蛋白外显子 蛋白质工作 

分 类 号：Q753[生物学—分子生物学] Q51