鹅细小病毒主要免疫原性蛋白基因的克隆与序列分析  

Cloning and Sequencing of the VP_3 Gene of Goose Parvovirus

作　　者：余兵[1] 王永坤[2] 刘宝荣[1] 朱国强[2] 严维巍[2] 王建业[2] 

机构地区：[1]淮阴检验检疫局,江苏淮安223001 [2]扬州大学动物医学系,江苏扬州225009

出　　处：《病毒学报》2002年第3期259-263,共5页Chinese Journal of Virology

摘　　要：利用PCR技术 ,从纯化鹅细小病毒SYG99-5毒株鹅胚尿囊液中扩增出病毒主要免疫原性蛋白基因。将该PCR扩增片段克隆入 pGEMR-T质粒载体的HincⅡ和SacⅠ位点之间 ,酶切分析筛选并进一步通过Southern杂交验证后 ,获得含 1 6kb基因片段的重组质粒GpG3 。序列测定结果表明 ,该片段与国外已报道的GPVB株苷酸序列有 96 %的同源性 ,氨基酸序列有Using PCR technique,VP 3 gene was amplified from the DNA of a field isolate of goose parvovirus(SYG 99-5 )isolated in China The PCR amplified VP 3 gene was cloned into the Hin cⅡ and SacⅠ sites of pGEM R-T Restriction endonuclease analysis and Southern-blotting showed that the recombinant plasmid GpG 3 containing 1 6kb fragment was obtained Sequence analysis showed that the Chinese isolate SYG 99-5 shared 96% and 97% identity with the sequence of foreign B strain of GPV at nucleotide and amino acid levels respectively

关 键 词：鹅细小病毒 免疫原性蛋白 克隆 序列分析 聚合酶链式反应 VP3基因 SOUTHERN杂交 重组质粒 

分 类 号：S852.659.2[农业科学—基础兽医学] S858.335.3[农业科学—兽医学]