除草剂乐草隆对红鲫的遗传毒性研究  

Genetic Toxicity in Lymphocyte of Red Crucian Carp Exposed to the Herbicide LeCaoLong

作　　者：张朝晖[1] 陈锋[1] 吴端生[2] 贺栋梁[1] 姚峰[2] 

机构地区：[1]南华大学公共卫生学院卫生学教研室,湖南421001 [2]南华大学实验动物学部

出　　处：《中国实验动物学报》2002年第3期185-187,F004,共4页Acta Laboratorium Animalis Scientia Sinica

基　　金：湖南省科技厅课题 (990 2 )

摘　　要：目的　探讨除草剂乐草隆对红鲫的遗传毒性。方法　用单细胞凝胶电泳检测不同浓度的乐草隆对红鲫外周血淋巴细胞DNA的损伤作用。结果　乐草隆致毒红鲫的淋巴细胞DNA的迁移度均较阴性对照组高 (P<0 0 5 ) ,在一定浓度范围内 (0～ 7 0 0mg L)DNA损伤程度与浓度呈正相关 (r=0 982 ,P <0 0 1)。在 12h、2 4h、4 8h、96h、10d实验组DNA损伤程度均有增强的趋势。Objective To study the effects of herbicide LeCaoLong on DNA damage of red crucian carp lymphocyte in vivo . Method Single cell gel electrophoresis (SCGE) assay was used to detect DNA single strands breaks (DNA SSBs) when red crucian carps were exposed to different concentration of LeCaoLong. Results At the concentration of 0 88, 1 75, 3 50, 7 00 mg/L, LeCaoLong could induce DNA damage in the peripheral blood lymphocyte of red curcian carp, the comet cell tail length of every group exposed to LeCaoLong was longer than the control group( P<0 05 ). The DNA damage was signficantly positively correlated with concentrations in the range of 0-7 0 mg/L,( r=0 982,P<0 01 ), when red crucian carps exposed to LeCaoLong for 12 h,24 h,48 h,96 h and 10days. The comet cell rates of every concentration group were higher than relative controls. Conclusion All the results suggest that the herbicide can cause significant genetic damage to the lymphocyte of red crucian carp at certain concentration and time.

关 键 词：除草剂 乐草隆 细鲫 遗传毒性 DNA损伤 鱼类 

分 类 号：X503.225[环境科学与工程—环境工程] S482.4[农业科学—农药学]