三种免疫制剂对真鲷弧菌病的免疫保护性  

THE IMMUNE PROTECTIVE EFFECTS OF THREE KINDS OF VACCINATION AGAINST VIBRIOSIS IN MARINE-CAGE CULTURED RED SEA BREAM(PAGRUS MAJOR)

作　　者：吴后波[1] 潘金培[1] 

机构地区：[1]中国科学院南海海洋研究所,广州510301

出　　处：《水生生物学报》2002年第5期457-464,共8页Acta Hydrobiologica Sinica

基　　金：中国科学院知识创新工程资助 (KSCX2 1 0 4) ;广东省"科技创新百项工程"资助项目 (99B0 62 0 1G) ;国家高技术研究发展计划 (2 0 0 1AA 62 2 0 5 0资助

摘　　要：菌体疫苗按不同的方式对真鲷进行免疫 2周后 ,对实验鱼均具有免疫保护性 ,免疫保护性最好的免疫组 ,免疫保护率在初次免疫后高达 60 % ,强化免疫后免疫保护率可提高到80 % ;粗制LPS经去毒处理后初次免疫真鲷 ,不同浓度的LPS对实验鱼具有不同程度的免疫保护性 ,强化免疫后 ,免疫保护率均有明显的提高 ,浓度越高 ,免疫保护性越强 ,对真鲷的免疫保护率最高可达 90 % ;最小弧菌产生的外毒素经福尔马林灭活后制成毒素苗 ,这种毒素苗能产生较好的免疫保护性 ,其免疫保护率可达 80 % ,这表明外毒素不仅是最小弧菌产生的毒力因子 。Red sea bream( Pagrus major ) were injected intramuscularly and direct immersion with Vibrio mimicus formalin killed cells (FKC), crude lipopolysaccharide(LPS)preparations and formalinized extracellular toxin(toxoid) of V. mimicus , respectively. The crude LPS prepared by the phenol water method had a little toxicity. The crude LPS was being heated to reduce its toxicity. The effectiveness of vaccination against Vibrio mimicus were evaluated two weeks after the immunization. The results showed that good protections were induced in red sea bream by FKC, LPS and toxoid. After booster immunization, the degrees of protection were enhanced.

关 键 词：免疫制剂 真鲷弧菌病 免疫保护性 菌体疫苗 粗制LPS 菌毒苗 

分 类 号：S941.42[农业科学—水产养殖]