HIV-1重组核酸疫苗质粒诱导小鼠免疫应答的研究  

Construction and immune response on nucleic vaccine plasmid of HIV-1_(CN) gp120 gene and IL-6 gene in mice

作　　者：郭焱[1] 金宁一[1] 张应玖[2] 郭志儒[1] 夏志平[1] 

机构地区：[1]解放军军需大学研究所病毒室,吉林长春130062 [2]吉林大学生命科学院生化教研室,吉林长春130021

出　　处：《免疫学杂志》2002年第5期340-342,346,共4页Immunological Journal

基　　金：国家自然科学基金 (39770 66);国家杰出青年基金 (3982 51 1 9)资助项目

摘　　要：目的　研究白细胞介素 6(IL 6)对人类免疫缺陷病毒 (HIV)gp1 2 0基因免疫小鼠的调节作用。方法　构建表达中国流行株HIV 1B亚型gp1 2 0基因的核酸疫苗质粒pGP及共表达中国流行株HIV 1B亚型gp1 2 0基因与IL 6基因的核酸疫苗质粒pGPIL 6 ,检测其在哺乳动物细胞中的表达并将上述两种质粒注射Balb/c鼠 ,检测小鼠产生的CD4+ 和CD8+ T细胞数情况及诱导CTL反应能力。结果　以间接免疫荧光法 (IFA)检测到gp1 2 0在哺乳动物细胞中得到表达。pGPIL 6免疫鼠 ,小鼠产生的CD4+ 和CD8+ T细胞数及诱导产生CTL反应的能力明显高于单独注射gp1 2 0的小鼠。结论　协同应用IL 6基因可明显加强HIV 1gp1 2 0基因免疫效果 ,为中国流行株HIVObjective To investigate the effect of interleukin 6 (IL 6) gene on the immune response to HIV gp120 gene. Methods Construction of nucleic vaccine plasmid of HIV 1 CN gp120 gene and IL 6 gene and expression were detected in mammalian cells .The two plasmids were co injected into tibilias anterior muscles of Balb/c mice.Results IFA showed that the constructed plasmids were successfully expressed in mammalian cells. Mice immunized with both gp120 gene and IL 6 gene exhibited a significant increase in number of CD4 +and CD8 +T and the CTL response compared to the mice immunized only with pGP.Conclusion HIV 1 CN gp120 antigen can be enhanced when HIV 1 CN gp120 gene and IL 6 gene are co injected.

关 键 词：人类免疫缺陷病毒 白细胞介素6 核酸疫苗质粒 艾滋病毒 AIDS疫苗 

分 类 号：R392[医药卫生—免疫学]