PPD对豚鼠实验性哮喘气道炎症的作用  

Effect of PPD on experimental asthma in a guinea pig model of allergen sensitization

作　　者：刘金保[1] 钟南山[1] 李树浓[2] 李晓宾[1] 梁仲培[1] 王桂房[1] 吴卫[1] 

机构地区：[1]广州医学院病理生理教研室,广东广州510182 [2]中山医科大学病理生理教研室,广东广州510089

出　　处：《免疫学杂志》2002年第5期350-352,共3页Immunological Journal

基　　金：国家自然科学基金 (3980 0 0 59);广东省自然科学基金 (980 4 60 )资助项目

摘　　要：目的　探索结核菌素纯蛋白衍生物 (purifiedproteinderivative ,PPD)对豚鼠实验性哮喘气道炎症的作用。方法　实验采用 31只豚鼠 ,体质量 2 50g左右 ,分为对照组、卵蛋白 (ovalbumin ,OVA)致敏组和PPD治疗组。用OVA(Ⅲ级 )致敏豚鼠复制豚鼠哮喘模型。结果　经过OVA致敏的动物气道支气管肺泡灌洗液 (BALF)和肺组织中嗜酸粒细胞以及BALF中白细胞总计数有明显增加 ,PPD可不同程度地降低肺组织嗜酸粒细胞的气道浸润 ,并使BALF中炎性细胞总数降低。Objective To examine the effect of PPD on airway inflammation induced by OVA sensitization.Methods Guinea pigs were sensitized with PPD and then with ovalbumin, challenged with ovalbumin aerosol inhalation.Results Ovalbumin inhalation caused a marked airway infiltration of eosinophils and all the animals exhibit asthmatic symptoms. PPD markedly inhibited eosinophil infiltration and attenuated the asthmatic symptoms. Conclusion It's concluded that PPD exerts protective effect on asthmatic inflammation in guinea pig model.

关 键 词：哮喘 豚鼠 气道炎症 实验研究 结核菌素纯蛋白衍生物 

分 类 号：R392[医药卫生—免疫学]