皮肤组织工程—细胞支架的构筑及其生物相容性评价  

Skin Tissue Engineering,Fabrication and Biocompatibility Evaluation of Cell Scaffold

作　　者：石桂欣[1] 王身国[1] 贝建中[1] 夏照帆[2] 肖仕初[2] 刘旺[2] 

机构地区：[1]中国科学院化学研究所分子科学中心,北京100080 [2]第二军医大学附属长海医院烧伤科,上海200433

出　　处：《北京生物医学工程》2002年第3期222-225,211,共5页Beijing Biomedical Engineering

基　　金：国家重点基础发展规划项目 (973计划G19990 5 43 0 5 ;G19990 5 43 0 6;G19990 5 43 0 9) ;上海市医学领先专业重点学科项目

摘　　要：皮肤组织工程的发展提供了一种无损伤修复创伤和功能重建的皮肤治疗模式。作为组织工程的三要素之一 ,细胞支架发挥着重要的作用。为满足组织工程中对细胞支架在力学性能、物理结构及生物相容性等方面的要求 ,我们首先制备了聚乳酸 (PDLLA)、聚乳酸 -己内酯 (PLACL)多孔支架 ,并以生物相容性较好的猪的无细胞真皮 (acellulardermismatrix ,ADM)为参比 ,分别把三种材料植入大鼠背部肌层 ,术后定期取大鼠皮下埋藏组织进行组织学检测。结果发现PDLLA与PLACL多孔支架的降解周期、力学性能、孔隙率及其孔径都可以根据皮肤组织工程中的要求进行调控。组织学检查 ,移植物内无明显炎性细胞 ,2 1天后 ,均完全血管化且分布较均匀。说明PDLLA与PLACL的生物相容性较ADM差 ,但并未出现明显的异物排斥反应 ,两者的生物相容性基本上可以满足组织工程中对支架的要求 。The development of skin tissue engineering provides a non invasive method for skin restoration.As one of three key factors in tissue engineering,the cell scaffolds play an important role.To meet the requirements of cell scaffolds for tissue engineering in respect of mechanical property,physical structure and biocompatibility,the porous scaffolds of poly(DL lactide)(PDLLA),and poly(lactide co caprolactone)(PLACL)were first fabricated,then they were implanted into the muscle of rat back.The rats died at different times after implantation and the retrieved implants from each rat were observed and compared with acellular dermis matrix (ADM)having good biocompatibility.It was found that the degradation rate,mechanical properties,porosity,and pore size of PDLLA and PLACL scaffolds can be adjusted according to the requirements of skin tissue engineering.There were no obvious inflammatory cells after implanting of the materials,and the formed vasa in the scaffolds became similar with normal vasa and distributed evenly after 21 days.The biocompatibility of PDLLA and PLACL is not as good as ADM,but the foreign body reactions were not obvious.The scaffolds of PDLLA and PLACL can meet elementary requirements of skin tissue engineering.This study provides meaningful and experimental basis for further study of artificial skin of PLACL.

关 键 词：皮肤组织工程 聚乳酸 聚乳酸-己内酯 细胞支架 生物相容性 

分 类 号：R322.99[医药卫生—人体解剖和组织胚胎学]