骨钙素放射免疫测定方法及其临床意义  

RADIOIMMUNOASSAY AND CIJNICAL SIGNIFICANCE OF OSTEOCALCIN

作　　者：孟昭亨[1] 沈海[1] 何玉香[1] 

机构地区：[1]北京创伤骨科研究所

出　　处：《中华内分泌代谢杂志》1991年第2期87-89,共3页Chinese Journal of Endocrinology and Metabolism

摘　　要：作者在国内首先制备了骨钙素(BGP)的抗血清,建立了BGP放射免疫测定方法。该抗血清特异性和灵敏度高,其最小检出量IC_(50)为2.5μg/L,批内差异<2.5％,批间差异<10.0％。应用本法测定了338例正常人和100例骨质疏松、糖尿病、卵巢切除、骨肿瘤、慢性肾功能衰竭、佝偻病和甲状旁腺功能亢进症患者血清BGP水平。骨质疏松BGP水平大多数下降,甲状旁腺功能亢进症、骨肿瘤者则明显升高,因此,血清BGP放免测定是骨病、代谢性骨病诊断和研究的一个重要检测手段。We produced BGP-antiserum, and developed the BGP RIA. This method was specific and sensitive for the detection of BGP. The intra-and inter-assay CV were less than 2.5% and 10% respectively. We determined BGP level in 338 normal subjects and 100 patients with various diseases, such as: osteoporosis, diabetes mellitus, post-ccphorectcmy, bone tumor, chronic renal failure,hyperparathyroidism and rickets. We found the BGP level in the patients with osteoporosis . was decreased, but those with hyperparathyroidism and bone tumor were significantly increased. So BGP RIA is an important method for diagnosing ostyeopathy and metabolic bone disease.

关 键 词：骨钙素 放射免疫测定 代谢性骨病 

分 类 号：R681.04[医药卫生—骨科学]