KF-950对人精子顶体素活性及顶体的影响  

Effect of Acrosin Inhibitor KF-950 on Acrosin Activity and Acrosome of Human Sperm

作　　者：詹瑛[1] 赵亚南 李宗林[3] 李丽 宋谦 

机构地区：[1]解放军401医院妇产科,山东青岛266071 [2]上海长海医院全军生殖医学研究中心,上海200433 [3]复旦大学生命科学院遗传研究所,上海200433

出　　处：《中华男科学杂志》2002年第2期92-94,共3页National Journal of Andrology

基　　金：上海市自然科学基金课题 (93 1960 790 )

摘　　要：目的 :研究顶体素抑制剂 4 胍基苯甲酸盐 (KF 95 0 )对人精子顶体素的抑制作用和对顶体的影响。　方法 :醋酸抽提并纯化人精子顶体素 ,BAEE/ADH法测定不同剂量KF 95 0作用后残余酶的活性。以生物素标记的豌豆凝集素为探针 ,采用ABC染色法观察KF 95 0对完整精子顶体的影响。　结果 :①正常人精子顶体素活性为(37.6 5± 4 .4 7)U/L(pH 8.5 ,2 5℃ ) ;②残余酶活性与KF 95 0剂量呈负相关 (r =- 0 .998) ,并呈量 效依赖关系 ,回归方程 :Y =7.5 7- 1.895X ;③随药物浓度的增加和作用时间的延长 ,顶体完整着色率均明显下降 (P <0 .0 1)。结论 :KF 95 0能直接抑制顶体素活性 ,推测对精子顶体也有明显的破坏作用 ,是一种新型、高效的精子顶体素抑制剂。Objectives: To study the inhibitory effect of KF 950 on human acrosin and sperm acrosome. Methods: Human acrosin was extracted and purified with 2% acetic acid,and its residual activity was evaluated by BAEE/ADH assay after treated with different concentrations of KF 950. ABC assay was used to observe the effect of KF 950 on human acrosome with Biotin PSA as a probe. Results: ①The activity of normal sperm acrosin was (37.65±4.47) U/L. ②The residual activity was inversely related to the concentration of KF 950 ( r =-0.998), and had a dose reponse curve.The result could be described by Y =7.57-1.895 X . ③With increase of KF 950 concentration and prolongation of action time, the staining rate of acrosome obviously dropped ( P <0.01). Conclusions: KF 950 directly inhibits acrosin activity and assumely injures sperm acrosome. It might be a new kind of highly effective inhibitor.

关 键 词：顶体素抑制剂 4胍基苯甲酸盐 顶体素 顶体 精子 

分 类 号：R321.1[医药卫生—人体解剖和组织胚胎学]