铁皮石斛组织培养繁殖技术研究  

Tissue Culture Technical of Dendrobium officinaleKimura et Migo

作　　者：郑慧俊[1] 张明丽[1] 邹春晶[1] 龚仲幸[1] 

机构地区：[1]杭州职业技术学院,杭州310018

出　　处：《农业工程》2012年第11期49-51,共3页AGRICULTURAL ENGINEERING

基　　金：杭州市农业发展基金扶持项目(铁皮石斛的引种筛选和栽培技术研究)

摘　　要：以铁皮石斛种荚为外植体,研究了不同消毒方式、培养基配方对于外植体生长的影响,探索最适于原球茎分化诱导、壮苗生长和生根的培养基配方。结果表明:有利于原球茎分化诱导的培养基配方是1/2 MS+2.0mg/L BA+0.2mg/L NAA;适合壮苗生长的培养基配方是MS+3.0mg/L BA+1.0mg/L NAA+1.0mg/L KT+3%香蕉;适合幼苗生根的培养基配方是1/2MS+0.5mg/L NAA+3%香蕉+0.5%活性碳。Taking seed pods of Dendrobium officinale Kimura et Migo as exophyte,effects of disinfection ways and medium formula on exophyte growth were studied.Optimum medium proportions for protocorm induction,strong seedlings growth and rootage were explored.Results showed that 1/2 MS+2.0 mg/L BA+0.2 mg/L NAA solid culture medium was advantageous to induce protocorm,MS+3.0 mg/L BA+1.0 mg/L NAA+1.0 mg/L KT+3% banana solid culture medium was suitable for strong seedling growth,and 1/2 MS+0.5mg/L NAA+3% banana+0.5% active carbon solid culture medium was the best medium to induce root.

关 键 词：铁皮石斛 组织培养 快速繁殖 培养基 

分 类 号：S567.239[农业科学—中草药栽培]