高糖对原代培养血管内皮细胞[Ca^(2+)]i和分泌LN及IV-C的影响  

Effect of high glucose on the expression of [Ca^(2+)]i and excretion LN and IV-C in HUVEC

作　　者：钟世军[1] 欧钢卫[1] 葛正龙[1] 

机构地区：[1]遵义医学院生物化学教研室,563003

出　　处：《贵州医药》2002年第9期774-776,共3页Guizhou Medical Journal

基　　金：贵州省教委科研基金资助项目

摘　　要：目的旨在探讨体外培养条件下高糖状态对血管内皮细胞的影响。方法以原代培养人脐带静脉内皮细胞为材料 ,用Fura 2荧光检测法观察高糖对原代培养血管内皮细胞内钙离子浓度([Ca2 + ]i)的影响 ,用放射免疫分析法观察高糖作用下血管内皮细胞分泌基底膜蛋白成分IV型胶原蛋白 (IV C)和层粘连蛋白 (LN)的情况。结果在 1 1 1 1mmol L、1 6 6 7mmol L和 33 33mmol L糖浓度下培养 2 4小时 ,血管内皮细胞 [Ca2 + ]i分别升高了 1 74、2 84和 6 74倍。发现不同糖浓度作用下血管内皮细胞分泌IV C的量均无变化 ;在 1 6 6 7mmol L糖浓度下培养 2 4小时血管内皮细胞分泌LN的量下降了 2 6 6 9%。结论在较短时间内糖浓度升高可引起血管内皮细胞 [Ca2 + ]i升高 ,而对血管内皮细胞分泌LN起抑制作用 ,对IV C的分泌无明显影响。在糖尿病微血管病变早期 ,高糖可能通过血管内皮细胞 [Ca2 +Objective To investigate the role of high glucose on human umbilical vein endothelial cell(HUVEC). Methods Fura 2/AM was used to determine the intracellular Ca 2+ concentration([Ca 2+ ]i) in HUVEC.Type IV collegan(IV C) and laminin(LN) which were secreted into media by HUVEC were determined with radioimmunoanalysis (RIA). Results The [Ca 2+ ]i in HUVEC treated by high glucose(11.11、16.67、 33.3 mmol/L of D glucose) for 24 hours,increased respectively 1.74,2.84,and 6.74 fold compared with normal control.However,the concentration of IV C in media,which was excreted by HUVEC treated by high glucose did not change.The level of LN in media decreased 26.69% compared with normal control.Conclusion The data suggest that [Ca 2+ ]i may play important role in early state of microvascular complications.

关 键 词：血管内皮细胞 细胞内钙 Ⅳ型胶原蛋白 层粘连蛋白 糖尿病 

分 类 号：R587.1[医药卫生—内分泌]